Literature summary for 3.4.23.5 extracted from

Follo, C.; Castino, R.; Nicotra, G.; Trincheri, N.F.; Isidoro, C.
Folding, activity and targeting of mutated human cathepsin D that cannot be processed into the double-chain form (2007), Int. J. Biochem. Cell Biol., 39, 638-649.

Search for more literature for 3.4.23.5

Application

Application	Comment	Organism
molecular biology	insights on the amino acid region involved in the terminal processing of human cathepsin D and on the function of the processing beta-hairpin loop are provided	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	a mutant cathepsin D protein is generated replacing the stretch from T155 to K175 (comprising the beta-hairpin loop) with the corresponding sequence S152-V159 of Clupea harengus. The mutant is successfully expressed in rodent cells (Neuro2A, L929 and DLD1 cells), reaches the lysosome and is stable as a single-chain peptide (47 kDa), bears high-mannose type sugars, binds to pepstatin A and is enzymatically active, indicating that it is correctly folded	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
pepstatin A	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	intermediate, single-chain CD	Homo sapiens
53000	-	precursor	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	human cathepsin D is synthesized as a di-glycosylated precursor of approximately 53 kDa that is processed by proteolysis first into an intermediate single-chain of 48 kDa and eventually into the mature double-chain form	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
using Pepstatin A-affinity purification	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Bovine hemoglobin + H2O	conjugated to the fluorochrome 6-((7-amino-4-methylcoumarin-3-acetyl) amino) hexanoic acid, succinimidyl ester	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin D	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.6	5	assay at	Homo sapiens

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Release 2023.1 (January 2023)