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Literature summary for 3.4.23.5 extracted from

  • Yasuda, Y.; Kohmura, K.; Kadowaki, T.; Tsukuba, T.; Yamamoto, K.
    A new selective substrate for cathepsin E based on the cleavage site sequence of alpha2-macroglobulin (2005), Biol. Chem., 386, 299-305.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by Ascaris pepsin inhibitor Rattus norvegicus
pepstatin A
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00212
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus
0.0022
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, spleen cathepsin D Rattus norvegicus
0.00265
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus
0.0037
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Rattus norvegicus
-
stomach
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O
-
Rattus norvegicus ?
-
?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O
-
Rattus norvegicus ?
-
?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 + H2O
-
Rattus norvegicus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.75
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus
2.2
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, spleen cathepsin D Rattus norvegicus
2.5
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus
59.6
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin D Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000027
-
pepstatin A 40°C, pH 4.0, gastric cathepsin D, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus
0.0000031
-
pepstatin A 40°C, pH 4.0, spleen cathepsin D, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus