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Literature summary for 3.4.23.5 extracted from
- Todarepsin, a new cathepsin D from hepatopancreas of Japanese common squid (Todarodes pacificus) (2004), Comp. Biochem. Physiol. B, 137, 373-382.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(p-nitrophenoxy)-propane | 4.5 mM, 68% residual activity | Todarodes pacificus |  |
| Diazoacetyl-DL-norleucine methyl ester | 0.05 mM, 66% residual activity | Todarodes pacificus | |
| additional information | not inhibitory: antipain, chymostatin, o-phenanthroline, phenylmethylsulfonylfluoride, iodoacetamide | Todarodes pacificus | |
| pepstatin A | 0.01 mM, no residual activity | Todarodes pacificus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
7-methoxycoumarin-4-yl-SEVNLDAEFRK(2,4-dinitrophenyl)RR | pH 2.2, 30°C | Todarodes pacificus | |
| 3.2 | - |
7-methoxycoumarin-4-yl-GKPILFFRLK(2,4-dinitrophenyl)-D-Arg | pH 2.2, 30°C | Todarodes pacificus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Todarodes pacificus |
| 36500 | - |
x * 36500, SDS-PAGE | Todarodes pacificus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Todarodes pacificus | - |
Japanese common squid | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Todarodes pacificus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Todarodes pacificus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-methoxycoumarin-4-yl-GKPILFFRLK(2,4-dinitrophenyl)-D-Arg + H2O | - |
Todarodes pacificus | ? | - |
? | |
| 7-methoxycoumarin-4-yl-SEVNLDAEFRK(2,4-dinitrophenyl)RR + H2O | - |
Todarodes pacificus | ? | - |
? | |
| acid-denatured casein + H2O | - |
Todarodes pacificus | ? | - |
? | |
| Acid-denatured hemoglobin + H2O | - |
Todarodes pacificus | ? | - |
? | |
| amyloid beta-protein + H2O | - |
Todarodes pacificus | ? | - |
? | |
| additional information | preferrence for hydrophobic amino acids at the scissile bond | Todarodes pacificus | ? | - |
? | |
| oxidized insulin beta-chain + H2O | - |
Todarodes pacificus | ? | - |
? | |
| renin substrate tetradecapeptide + H2O | - |
Todarodes pacificus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 36500, SDS-PAGE | Todarodes pacificus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapid loss of activity | Todarodes pacificus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
substrate acid-denatured casein | Todarodes pacificus |
| 3 | - |
substrate 7-methoxycoumarin-4-yl-GKPILFFRLK(2,4-dinitrophenyl)-D-Arg | Todarodes pacificus |
| 3.5 | - |
substrate acid-denatured hemoglobin | Todarodes pacificus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000005 | - |
pepstatin A | pH 2.2, 30°C | Todarodes pacificus | |
| 0.2 | - |
1,2-epoxy-3-(p-nitrophenoxy)-propane | pH 2.2, 30°C | Todarodes pacificus | |
| 0.5 | - |
Diazoacetyl-DL-norleucine methyl ester | pH 2.2, 30°C | Todarodes pacificus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Todarodes pacificus | isoelectric focusing | - |
8.29 |
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