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Literature summary for 3.4.23.5 extracted from

  • Doke, S.N.; Ninjoor, V.; Nadkarni, G.B.
    Characterization of cathepsin D from the skeletal muscle of fresh water fish, Tilapia mossambica (1980), Agric. Biol. Chem., 44, 1521-1528.
No PubMed abstract available
Search for more literature for 3.4.23.5

Inhibitors

Inhibitors Comment Organism Structure
NaCl 10%, almost complete inactivation Tilapia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Tilapia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Tilapia sp.

Organism

Organism UniProt Comment Textmining
Tilapia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tilapia sp.

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Tilapia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0385
-
-
 Tilapia sp.

Storage Stability

Storage Stability Organism
-10°C, purified enzyme dialyzed against distilled water, 40% loss of activity after 3 months Tilapia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O denatured hemoglobin Tilapia sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Tilapia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: about 65% of maximal activity, 60°C: about 85% of maximal activity Tilapia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20% loss of activity after 10 min, 35% loss of activity after 60 min Tilapia sp.
60
-
 stable in presence of substrate, 95% inactivation after 60 min, in absence of substrate Tilapia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8
-
 and a second optimum at pH 3.8, denatured hemoglobin Tilapia sp.
3.8
-
 and a second optimum at pH 2.8, denatured hemoglobin Tilapia sp.
5
-
 degradation of endogenous sarcoplasmic proteins Tilapia sp.