Cloned (Comment) | Organism |
---|---|
inserted into pTrc99A, expressed in Escherichia coli, additional strains and plasmids of the study listed | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli | 19867 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
colicin E1 + H2O | Escherichia coli | function in degradation of colicin at the cell surface to protect sensitive cells from infection by colicins suggested | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09169 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, gel filtration and SDS-PAGE | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
strains expressing OmpT protease shown to cleave colicin E1 at the residues K84 and K95 in the N-terminal translocation domain, leading to the removal of the TolQA box essential for cytotoxicity of colicin E1, in vivo data indicating effects of OmpT protease on colicin E1 cell-killing activity shown | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colicin E1 + H2O | function in degradation of colicin at the cell surface to protect sensitive cells from infection by colicins suggested | Escherichia coli | ? | - |
? | |
colicin E1 + H2O | cleavage by involvement of OmpT proteases determined by SDS-PAGE, processing site determined | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OmpT protease | - |
Escherichia coli |