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Literature summary for 3.4.23.49 extracted from

  • Masi, M.; Vuong, P.; Humbard, M.; Malone, K.; Misra, R.
    Initial steps of colicin E1 import across the outer membrane of Escherichia coli (2007), J. Bacteriol., 189, 2667-2676.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
inserted into pTrc99A, expressed in Escherichia coli, additional strains and plasmids of the study listed Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane
-
Escherichia coli 19867
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
colicin E1 + H2O Escherichia coli function in degradation of colicin at the cell surface to protect sensitive cells from infection by colicins suggested ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09169
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, gel filtration and SDS-PAGE Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
strains expressing OmpT protease shown to cleave colicin E1 at the residues K84 and K95 in the N-terminal translocation domain, leading to the removal of the TolQA box essential for cytotoxicity of colicin E1, in vivo data indicating effects of OmpT protease on colicin E1 cell-killing activity shown Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colicin E1 + H2O function in degradation of colicin at the cell surface to protect sensitive cells from infection by colicins suggested Escherichia coli ?
-
?
colicin E1 + H2O cleavage by involvement of OmpT proteases determined by SDS-PAGE, processing site determined Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
OmpT protease
-
Escherichia coli