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Literature summary for 3.4.23.49 extracted from

  • Kramer, R.A.; Zandwijken, D.; Egmond, M.R.; Dekker, N.
    In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT (2000), Eur. J. Biochem., 267, 885-893.
    View publication on PubMed
Search for more literature for 3.4.23.49

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed without its signal sequence in Escherichia coli K-12 strain DH5alpha using a T7 system Escherichia coli

Protein Variants

Protein Variants Comment Organism
G216K/K217G recombinant ompT variant in order to abolish autoproteolysis Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate significant inhibition only at high concentrations Escherichia coli
phenylmethylsulfonyl fluoride poor inhibitor, only at high concentrations Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
0.0004
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, recombinant expressed Escherichia coli
0.0011
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, variant G216K/K217G Escherichia coli
0.53
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
0.58
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, recombinant expressed Escherichia coli
0.79
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, variant G216K/K217G Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protease, outer membrane, membrane bound Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Escherichia coli
33480
-
 ESI-MS,positive-ion-mode electrospray ionization mass spectrometry Escherichia coli
33500
-
 calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli cleaves peptides between two consecutive basic amino acids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli 2-aminobenzoyl-Ile-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 ?
aminobenzoyl-Ala-Lys-Lys-Ala-3-(dinitrophenyl)diaminopropionic acid-Gly + H2O
-
 Escherichia coli ?
-
 ?
IAA-Arg-Arg-p-nitroanilide + H2O
-
 Escherichia coli ?
-
 ?
additional information cleaves peptides between two consecutive basic amino acids Escherichia coli ?
-
 ?
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
ompT
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, variant G216K/K217G Escherichia coli
24
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, purified from outer membrane and wild-type, recombinant expressed Escherichia coli
96
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, variant G216K/K217G Escherichia coli
2280
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
2460
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, recombinant expressed in Escherichia coli Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Escherichia coli