Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.49 extracted from

  • Dekker, N.; Cox, R.C.; Kramer, R.A.; Egmond, M.R.
    Substrate specificity of the integral membrane protease ompT determined by spatially addressed peptide libraries (2001), Biochemistry, 40, 1694-1701.
    View publication on PubMed
Search for more literature for 3.4.23.49

Inhibitors

Inhibitors Comment Organism Structure
2-aminobenzoyl-A-(L)R-(D)R-A-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 Escherichia coli
phenylmethylsulfonyl fluoride
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 2-aminobenzoyl-RRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
0.0031
-
 2-aminobenzoyl-ARRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
0.0036
-
 2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
0.0065
-
 RRLELYKRHHG pH 7.0, 25°C Escherichia coli
0.0067
-
 RRELRLYRRHHG pH 8.3, 37°C Escherichia coli
0.0083
-
 2-aminobenzoyl-AKRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
0.0104
-
 2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
0.052
-
 2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protease, outer membrane, membrane bound Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Escherichia coli
180000
-
 macromolecular complex Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
2-aminobenzoyl-AKRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
2-aminobenzoyl-ARRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli 2-aminobenzoyl-Ala-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 ?
2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli 2-aminobenzoyl-Ile-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 ?
2-aminobenzoyl-RRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli 2-aminobenzoyl-L-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 ?
acetyl-Ala-Lys-(D)Arg-Val-Gly-(beta)-Ala + H2O
-
 Escherichia coli ?
-
 ?
aminobenzoyl-Ala-Lys-Lys-Ala-3-(dinitrophenyl)diaminopropionic acid-Gly + H2O
-
 Escherichia coli ?
-
 ?
aminobenzoyl-ARRA-Tyr(NO2)-G + H2O
-
 Escherichia coli ?
-
 ?
additional information little or no reaction with aminobenzoylfluorophore-ARIA-(dinitrophenyl)diaminopropionic acid quencher-G and aminobenzoylfluorophore-ARRIA-3-(dinitrophenyl)diaminopropionic acid quencher-G, acetyl-3-(dinitrophenyl)diaminopropionic acid-Ala-Arg-Arg-Ala-Lys(aminobenzoyl)-Gly is no substrate, no hydrolytic activity toward aminobenzoylfluorophore-A-(D)R-(L)R-A-3-(dinitrophenyl)diaminopropionic acid quencher-G, aminobenzoylfluorophore-A-(L)R-(D)R-A-3-(dinitrophenyl)diaminopropionic acid quencher-G and aminobenzoylfluorophore-A-(D)R-(D)R-A-3-(dinitrophenyl)diaminopropionic acid quencher-G Escherichia coli ?
-
 ?
RRELRLYRRHHG + H2O
-
 Escherichia coli ?
-
 ?
RRLELYKRHHG + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
ompT
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
78
-
 2-aminobenzoyl-RRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
84
-
 2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
192
-
 2-aminobenzoyl-ARRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
612
-
 2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
738
-
 2-aminobenzoyl-AKRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
2380
-
 2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli
2400
-
 aminobenzoyl-Ala-Lys-Lys-Ala-3-(dinitrophenyl)diaminopropionic acid-Gly pH 8.3, 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
 2-aminobenzoyl-A-(L)R-(D)R-A-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly pH 8.3, 37°C Escherichia coli