Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | - |
Escherichia coli | |
benzamidine | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
diisopropylfluorophosphate | - |
Escherichia coli | |
Fe2+ | - |
Escherichia coli | |
NaCl | optimal activity in absence of NaCl, 80% inhibition by 0.15 M NaCl | Escherichia coli | |
Zn2+ | uncompetitive, pH-dependent; ZnCl2 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000058 | - |
plasminogen | pH 5.0 | Escherichia coli | |
0.0036 | - |
plasminogen | pH 7.4 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli | 19867 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | cleavage at an Arg-Val bond | Escherichia coli | heavy and light chain of plasmin + ? | - |
? | |
Recombinant human gamma-interferon + H2O | cleavage between basic amino acids | Escherichia coli | ? | - |
? | |
T7 RNA polymerase + H2O | cleavage at Lys-Arg173, Lys-Lys180, and Arg-Lys392 | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | 37°C: 40% of maximal activity, 45°C: maximal activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
plasminogen | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | 4: 95% of maximal activity, 6.0: 25% of maximal activity | Escherichia coli |