Cloned (Comment) | Organism |
---|---|
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequence comparisons | Yersinia pestis subsp. pestis |
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequencing of pla genes from 118 Yersinia pestis subsp. microtus isolates, sequence comparisons | Yersinia pestis subsp. microtus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the Pla I259T modification is not required to cause pneumonic plague in the murine intranasal model | Yersinia pestis subsp. pestis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
plasminogen activator inhibitor 1 | PAI-1 | Yersinia pestis subsp. microtus | |
plasminogen activator inhibitor 1 | PAI-1 | Yersinia pestis subsp. pestis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis subsp. microtus | A0A141BQM9 | SNP-types 0.PE3 and 0.PE4 | - |
Yersinia pestis subsp. microtus 91001 | A0A141BQM9 | SNP-types 0.PE3 and 0.PE4 | - |
Yersinia pestis subsp. pestis | P17811 | T259 isotype | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme performs autoproteolysis | Yersinia pestis subsp. microtus |
proteolytic modification | the enzyme performs autoproteolysis | Yersinia pestis subsp. pestis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O | - |
Yersinia pestis subsp. microtus | (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 | - |
? | |
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O | - |
Yersinia pestis subsp. pestis | (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 | - |
? | |
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O | - |
Yersinia pestis subsp. microtus 91001 | (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 | - |
? | |
additional information | the enzyme performs autoproteolysis | Yersinia pestis subsp. microtus | ? | - |
? | |
additional information | the enzyme performs autoproteolysis | Yersinia pestis subsp. pestis | ? | - |
? | |
additional information | the enzyme performs autoproteolysis | Yersinia pestis subsp. microtus 91001 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34100, SDS-PAGE, x * 32600, about, unprocessed enzyme, sequence calculation, x * 29600, about, processed enzyme, sequence calculation | Yersinia pestis subsp. pestis |
? | x * 36900, SDS-PAGE, x * 32600, about, unprocessed enzyme, sequence calculation, x * 29600, about, processed enzyme, sequence calculation | Yersinia pestis subsp. microtus |
More | the Pla peptide has an atypical mobility on the protein gel | Yersinia pestis subsp. microtus |
More | the Pla peptide has an atypical mobility on the protein gel | Yersinia pestis subsp. pestis |
Synonyms | Comment | Organism |
---|---|---|
coagulase | - |
Yersinia pestis subsp. microtus |
coagulase | - |
Yersinia pestis subsp. pestis |
fibrinolysin | - |
Yersinia pestis subsp. microtus |
fibrinolysin | - |
Yersinia pestis subsp. pestis |
Pla | - |
Yersinia pestis subsp. microtus |
Pla | - |
Yersinia pestis subsp. pestis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Yersinia pestis subsp. microtus |
37 | - |
assay at | Yersinia pestis subsp. pestis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Yersinia pestis subsp. microtus |
7.4 | - |
assay at | Yersinia pestis subsp. pestis |
General Information | Comment | Organism |
---|---|---|
evolution | the modern isoform of Pla (T259) with an increased protease activity is found in Yersinia pestis subsp. pestis strains that are highly virulent for humans. The pathogenicity factor is absent in representatives of the Yersinia pestis subsp. microtus bv. caucasica/SNP-type 0.PE2, while an ancestral Pla isoform (I259) with characteristics similar to the properties of omptins from less virulent enterobacteria is found in three representatives of Yersinia pestis subsp. microtus (SNP-types 0.PE3 and 0.PE4), which are, as a rule, avirulent to guinea pigs and humans. Distribution of Pla isoforms among natural isolates of Yersinia pestis of different origin, overview | Yersinia pestis subsp. pestis |
evolution | the pathogenicity factor is absent in representatives of the Yersinia pestis subsp. microtus bv. caucasica/SNP-type 0.PE2, while an ancestral Pla isoform (I259) with characteristics similar to the properties of omptins from less virulent enterobacteria is found in three representatives of Yersinia pestis subsp. microtus (SNP-types 0.PE3 and 0.PE4), which are, as a rule, avirulent to guinea pigs and humans. The modern isoform of Pla (T259) with an increased protease activity is found in Yersinia pestis subsp. pestis strains that are highly virulent for humans. Sequencing of pla genes from 118 Yersinia pestis subsp. microtus isolates reveals the absence of this gene in the strains belonging to bv. caucasica. All remaining isolates of Yersinia pestis subsp. microtus contain the ancestral Pla isoform (I259). Distribution of Pla isoforms among natural isolates of Yersinia pestis of different origin, overview | Yersinia pestis subsp. microtus |
additional information | the primary action of Pla is to protect bacteria from destruction rather than to alter the tissue environment to favor Yersinia pestis propagation in the host. Analysis of intraspecies distribution, intrinsic disorder propensity, and contribution to virulence of the two isoforms of Yersinia pestis plasminogen activator Pla, overview. Survival curves of the endemic I259 Pla+ strains are similar to the parent Pla-negative variants, but significant difference in mean time to death post infection between the Pla- strains and their I259 Pla+ variants can be seen only in the isogenic set of Yersinia pestis subsp. pestis strains. An essential role for the outer membrane protease Pla evolution in Yersinia pestis bubonic infection exacerbation is suggested that is necessary for intensification of epidemic process from endemic natural focality with sporadic cases in humans to rapidly expanding epizootics followed by human epidemic outbreaks, local epidemics or even pandemics. Pla expression is associated with a marked ability to colonize the viscera and thus cause lethal infection upon administration by peripheral routes of infection, such as intradermal or subcutaneous. The Pla activity is not required to initiate lethal disease by intravenous injection, which provides immediate access to fixed macrophages lining the capillary beds of the liver and spleen. Virulence study of Yersinia pestis expressing different isoforms of Pla in a bubonic plague model | Yersinia pestis subsp. microtus |
additional information | the primary action of Pla is to protect bacteria from destruction rather than to alter the tissue environment to favor Yersinia pestis propagation in the host. Analysis of intraspecies distribution, intrinsic disorder propensity, and contribution to virulence of the two isoforms of Yersinia pestis plasminogen activator Pla, overview. Survival curves of the endemic I259 Pla+ strains are similar to the parent Pla-negative variants, but significant difference in mean time to death post infection between the Pla- strains and their I259 Pla+ variants can be seen only in the isogenic set of Yersinia pestis subsp. pestis strains. Pla expression is associated with a marked ability to colonize the viscera and thus cause lethal infection upon administration by peripheral routes of infection, such as intradermal or subcutaneous. The Pla activity is not required to initiate lethal disease by intravenous injection, which provides immediate access to fixed macrophages lining the capillary beds of the liver and spleen. Virulence study of Yersinia pestis expressing different isoforms of Pla in a bubonic plague model | Yersinia pestis subsp. pestis |