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Literature summary for 3.4.23.48 extracted from

  • Vranckx, L.; De Buck, E.; Anne, J.; Lammertyn, E.
    Legionella pneumophila exhibits plasminogen activator activity (2007), Microbiology, 153, 3757-3765.
    View publication on PubMed
Search for more literature for 3.4.23.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TG1, strains BL21 and BL21(DE3)pLys used for expression of recombinant proteins Legionella pneumophila

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane outer membrane protein Legionella pneumophila 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Legionella pneumophila capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined plasmin + ?
-
 ?
plasminogen + H2O Legionella pneumophila Philadelphia capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined plasmin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila
-
 intracellularly grown in Acanthamoeba castellanii ATCC 30234
-
Legionella pneumophila Philadelphia
-
 intracellularly grown in Acanthamoeba castellanii ATCC 30234
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, gel fitration Legionella pneumophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 expression of Lpa gene shown in exponential and stationary growth phase, transcription shown to be directed by own promoter Legionella pneumophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Val-Leu-Lys-p-nitroanilide + H2O Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured Legionella pneumophila D-Val-Leu-Lys + p-nitroaniline
-
 ?
D-Val-Leu-Lys-p-nitroanilide + H2O Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured Legionella pneumophila Philadelphia D-Val-Leu-Lys + p-nitroaniline
-
 ?
plasminogen + H2O capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined Legionella pneumophila plasmin + ?
-
 ?
plasminogen + H2O capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined Legionella pneumophila Philadelphia plasmin + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 plasminogen activity assay Legionella pneumophila