BRENDA - Enzyme Database show
show all sequences of 3.4.23.48

Legionella pneumophila exhibits plasminogen activator activity

Vranckx, L.; De Buck, E.; Anne, J.; Lammertyn, E.; Microbiology 153, 3757-3765 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli TG1, strains BL21 and BL21(DE3)pLys used for expression of recombinant proteins
Legionella pneumophila
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
outer membrane protein
Legionella pneumophila
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
plasminogen + H2O
Legionella pneumophila
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
plasmin + ?
-
-
?
plasminogen + H2O
Legionella pneumophila Philadelphia
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
plasmin + ?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Legionella pneumophila
-
intracellularly grown in Acanthamoeba castellanii ATCC 30234
-
Legionella pneumophila Philadelphia
-
intracellularly grown in Acanthamoeba castellanii ATCC 30234
-
Purification (Commentary)
Commentary
Organism
recombinant protein, gel fitration
Legionella pneumophila
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
expression of Lpa gene shown in exponential and stationary growth phase, transcription shown to be directed by own promoter
Legionella pneumophila
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Val-Leu-Lys-p-nitroanilide + H2O
Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured
683853
Legionella pneumophila
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
D-Val-Leu-Lys-p-nitroanilide + H2O
Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured
683853
Legionella pneumophila Philadelphia
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
plasminogen + H2O
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
683853
Legionella pneumophila
plasmin + ?
-
-
-
?
plasminogen + H2O
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
683853
Legionella pneumophila Philadelphia
plasmin + ?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
plasminogen activity assay
Legionella pneumophila
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli TG1, strains BL21 and BL21(DE3)pLys used for expression of recombinant proteins
Legionella pneumophila
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
outer membrane protein
Legionella pneumophila
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
plasminogen + H2O
Legionella pneumophila
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
plasmin + ?
-
-
?
plasminogen + H2O
Legionella pneumophila Philadelphia
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
plasmin + ?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein, gel fitration
Legionella pneumophila
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
expression of Lpa gene shown in exponential and stationary growth phase, transcription shown to be directed by own promoter
Legionella pneumophila
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Val-Leu-Lys-p-nitroanilide + H2O
Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured
683853
Legionella pneumophila
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
D-Val-Leu-Lys-p-nitroanilide + H2O
Lpa gene encoding a homologue of the plasminogen activator Pla of Yersinia pestis identified, described as a surface protease exhibiting plasminogen activator activity, plasminogen activity of inner and outer membranes measured
683853
Legionella pneumophila Philadelphia
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
plasminogen + H2O
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
683853
Legionella pneumophila
plasmin + ?
-
-
-
?
plasminogen + H2O
capacity to convert plasminogen into plasmin by the action of the outer-membrane Lpa protein determined
683853
Legionella pneumophila Philadelphia
plasmin + ?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
plasminogen activity assay
Legionella pneumophila
Other publictions for EC 3.4.23.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734631
van Lier
Further characterization of a ...
Yersinia pestis
Microb. Pathog.
80
27-38
2015
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1
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1
1
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734331
Ruback
Structural analysis of Pla pro ...
Yersinia pestis
J. Biomol. Struct. Dyn.
31
477-484
2013
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1
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734459
Lane
Proteolytic processing of the ...
Yersinia pestis
J. Med. Microbiol.
62
1124-1134
2013
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1
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734713
Lawrenz
Acquisition of omptin reveals ...
Yersinia pestis
Mol. Microbiol.
89
276-287
2013
-
-
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1
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1
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1
1
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735081
Simon
Fast and simple detection of Y ...
Yersinia pestis
PLoS ONE
8
e54947
2013
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1
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734184
Eren
Structural basis for activatio ...
Yersinia pestis
J. Biol. Chem.
287
23971-23976
2012
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717438
Haiko
Molecular adaptation of a plan ...
Yersinia pestis
BMC Evol. Biol.
11
43
2011
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1
1
1
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1
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3
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712153
Tsang
Ail binding to fibronectin fac ...
Yersinia pestis
Infect. Immun.
78
3358-3368
2010
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1
1
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718407
Eren
An active site water network i ...
Yersinia pestis
Structure
18
809-818
2010
-
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1
1
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695656
Matero
Real-time multiplex PCR assay ...
Yersinia pestis
APMIS
117
34-44
2009
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712282
Haiko
The single substitution I259T, ...
Yersinia pestis
J. Bacteriol.
191
4758-4766
2009
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1
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1
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2
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2
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5
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2
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2
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713437
Feodorova
-
Yersinia pestis live vaccine w ...
Yersinia pestis
Procedia Vaccinol.
1
97-100
2009
-
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1
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683251
Agarkov
Substrate specificity and scre ...
Yersinia pestis
Bioorg. Med. Chem. Lett.
18
427-431
2008
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1
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698832
Zhang
Plasminogen activator Pla of Y ...
Yersinia pestis
J. Biol. Chem.
283
31511-31521
2008
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7
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683853
Vranckx
Legionella pneumophila exhibit ...
Legionella pneumophila, Legionella pneumophila Philadelphia
Microbiology
153
3757-3765
2007
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684061
Lathem
A plasminogen-activating prote ...
Yersinia pestis
Science
315
509-513
2007
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668672
Lobo
Adhesive properties of the pur ...
Yersinia pestis
FEMS Microbiol. Lett.
262
158-162
2006
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668991
Benedek
Identification of laminin-bind ...
Yersinia pestis
Int. J. Med. Microbiol.
295
87-98
2005
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2
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669113
Chromy
Proteomic characterization of ...
Yersinia pestis
J. Bacteriol.
187
8172-8180
2005
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668759
Korhonen
-
Plasminogen activator of Yersi ...
Yersinia pestis
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
1
217-219
2004
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1
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1
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1
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5
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1
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670148
Benedek
Intracellular signalling and c ...
Yersinia pestis
Microb. Pathog.
37
47-54
2004
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638926
Benedek
Mapping of possible laminin bi ...
Yersinia pestis
Adv. Exp. Med. Biol.
529
101-104
2003
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638923
Lahteenmaki
The Pla surface protease/adhes ...
Yersinia pestis
FEBS Lett.
504
69-72
2001
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1
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2
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6
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1
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638924
Kukkonen
Protein regions important for ...
Yersinia pestis
Mol. Microbiol.
40
1097-1111
2001
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25
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6
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1
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25
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1
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638925
Lahteenmaki
Bacterial plasminogen activato ...
Mammalia, Mycobacterium tuberculosis, Streptococcus uberis, Yersinia pestis
FEMS Microbiol. Rev.
25
531-552
2001
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4
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4
1
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4
1
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638922
Kutyrev
Expression of the plague plasm ...
Yersinia pestis
Infect. Immun.
67
1359-1367
1999
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7
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638921
Lahteenmaki
Expression of plasminogen acti ...
Yersinia pestis
Infect. Immun.
66
5755-5762
1998
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3
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5
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638920
Kienle
-
Adhesive properties conferred ...
Yersinia pestis
J. Gen. Microbiol.
138
1679-1687
1992
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