BRENDA - Enzyme Database show
show all sequences of 3.4.23.48

Protein regions important for plasminogen activation and inactivation of a2-antiplasmin in the surface protease Pla of Yersinia pestis

Kukkonen, M.; Lahteenmaki, K.; Suomalainen, M.; Kalkkinen, N.; Emody, L.; Lang, H.; Korhonen, T.K.; Mol. Microbiol. 40, 1097-1111 (2001)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D206A
1.3% of wild-type plasminogen activation
Yersinia pestis
D212R
111% of wild-type plasminogen activation
Yersinia pestis
D84A
3.7% of wild-type plasminogen activation
Yersinia pestis
D86A
3.5% of wild-type plasminogen activation
Yersinia pestis
D97A
56% of wild-type plasminogen activation
Yersinia pestis
E217S
52% of wild-type plasminogen activation
Yersinia pestis
F215Y
42% of wild-type plasminogen activation
Yersinia pestis
H101V
0.8% of wild-type plasminogen activation
Yersinia pestis
H108V
121% of wild-type plasminogen activation
Yersinia pestis
H203V
109% of wild-type plasminogen activation
Yersinia pestis
H208V
1.7% of wild-type plasminogen activation
Yersinia pestis
H28V
125% of wild-type plasminogen activation
Yersinia pestis
H98V
94% of wild-type plasminogen activation
Yersinia pestis
K218A
103% of wild-type plasminogen activation
Yersinia pestis
K240A
151% of wild-type plasminogen activation
Yersinia pestis
K249A
132% of wild-type plasminogen activation
Yersinia pestis
K254A
90% of wild-type plasminogen activation
Yersinia pestis
K262A
85% of wild-type plasminogen activation
Yersinia pestis
K280A
98% of wild-type plasminogen activation
Yersinia pestis
L213I
123% of wild-type plasminogen activation
Yersinia pestis
M210G
58% of wild-type plasminogen activation
Yersinia pestis
R211K
11% of wild-type plasminogen activation
Yersinia pestis
S267A
108% of wild-type plasminogen activation
Yersinia pestis
S77A
133% of wild-type plasminogen activation
Yersinia pestis
S99A
1% of wild-type plasminogen activation
Yersinia pestis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
human alpha2-antiplasmin + H2O
Yersinia pestis
-
fragments of alpha2-antiplasmin
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Yersinia pestis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
human alpha2-antiplasmin + H2O
-
638924
Yersinia pestis
fragments of alpha2-antiplasmin
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
Pla occurs in 3 processed forms of alpha-Pla, beta-Pla and gamma-Pla, of which alpha-Pla with an apparant molecular mass of 37000 Da, corresponds to full-size mature Pla, and beta-Pla and gamma-Pla with molecular weights of 31000 Da and 35000 Da respectively, are thought to result from processing of alpha-Pla, beta-Pla may result from an autoprocessing event
Yersinia pestis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D206A
1.3% of wild-type plasminogen activation
Yersinia pestis
D212R
111% of wild-type plasminogen activation
Yersinia pestis
D84A
3.7% of wild-type plasminogen activation
Yersinia pestis
D86A
3.5% of wild-type plasminogen activation
Yersinia pestis
D97A
56% of wild-type plasminogen activation
Yersinia pestis
E217S
52% of wild-type plasminogen activation
Yersinia pestis
F215Y
42% of wild-type plasminogen activation
Yersinia pestis
H101V
0.8% of wild-type plasminogen activation
Yersinia pestis
H108V
121% of wild-type plasminogen activation
Yersinia pestis
H203V
109% of wild-type plasminogen activation
Yersinia pestis
H208V
1.7% of wild-type plasminogen activation
Yersinia pestis
H28V
125% of wild-type plasminogen activation
Yersinia pestis
H98V
94% of wild-type plasminogen activation
Yersinia pestis
K218A
103% of wild-type plasminogen activation
Yersinia pestis
K240A
151% of wild-type plasminogen activation
Yersinia pestis
K249A
132% of wild-type plasminogen activation
Yersinia pestis
K254A
90% of wild-type plasminogen activation
Yersinia pestis
K262A
85% of wild-type plasminogen activation
Yersinia pestis
K280A
98% of wild-type plasminogen activation
Yersinia pestis
L213I
123% of wild-type plasminogen activation
Yersinia pestis
M210G
58% of wild-type plasminogen activation
Yersinia pestis
R211K
11% of wild-type plasminogen activation
Yersinia pestis
S267A
108% of wild-type plasminogen activation
Yersinia pestis
S77A
133% of wild-type plasminogen activation
Yersinia pestis
S99A
1% of wild-type plasminogen activation
Yersinia pestis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
human alpha2-antiplasmin + H2O
Yersinia pestis
-
fragments of alpha2-antiplasmin
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
human alpha2-antiplasmin + H2O
-
638924
Yersinia pestis
fragments of alpha2-antiplasmin
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
Pla occurs in 3 processed forms of alpha-Pla, beta-Pla and gamma-Pla, of which alpha-Pla with an apparant molecular mass of 37000 Da, corresponds to full-size mature Pla, and beta-Pla and gamma-Pla with molecular weights of 31000 Da and 35000 Da respectively, are thought to result from processing of alpha-Pla, beta-Pla may result from an autoprocessing event
Yersinia pestis
Other publictions for EC 3.4.23.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734631
van Lier
Further characterization of a ...
Yersinia pestis
Microb. Pathog.
80
27-38
2015
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1
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734331
Ruback
Structural analysis of Pla pro ...
Yersinia pestis
J. Biomol. Struct. Dyn.
31
477-484
2013
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734459
Lane
Proteolytic processing of the ...
Yersinia pestis
J. Med. Microbiol.
62
1124-1134
2013
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734713
Lawrenz
Acquisition of omptin reveals ...
Yersinia pestis
Mol. Microbiol.
89
276-287
2013
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1
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735081
Simon
Fast and simple detection of Y ...
Yersinia pestis
PLoS ONE
8
e54947
2013
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1
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734184
Eren
Structural basis for activatio ...
Yersinia pestis
J. Biol. Chem.
287
23971-23976
2012
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717438
Haiko
Molecular adaptation of a plan ...
Yersinia pestis
BMC Evol. Biol.
11
43
2011
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1
1
1
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3
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712153
Tsang
Ail binding to fibronectin fac ...
Yersinia pestis
Infect. Immun.
78
3358-3368
2010
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1
1
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718407
Eren
An active site water network i ...
Yersinia pestis
Structure
18
809-818
2010
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1
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695656
Matero
Real-time multiplex PCR assay ...
Yersinia pestis
APMIS
117
34-44
2009
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712282
Haiko
The single substitution I259T, ...
Yersinia pestis
J. Bacteriol.
191
4758-4766
2009
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5
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713437
Feodorova
-
Yersinia pestis live vaccine w ...
Yersinia pestis
Procedia Vaccinol.
1
97-100
2009
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683251
Agarkov
Substrate specificity and scre ...
Yersinia pestis
Bioorg. Med. Chem. Lett.
18
427-431
2008
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698832
Zhang
Plasminogen activator Pla of Y ...
Yersinia pestis
J. Biol. Chem.
283
31511-31521
2008
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6
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683853
Vranckx
Legionella pneumophila exhibit ...
Legionella pneumophila, Legionella pneumophila Philadelphia
Microbiology
153
3757-3765
2007
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684061
Lathem
A plasminogen-activating prote ...
Yersinia pestis
Science
315
509-513
2007
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668672
Lobo
Adhesive properties of the pur ...
Yersinia pestis
FEMS Microbiol. Lett.
262
158-162
2006
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668991
Benedek
Identification of laminin-bind ...
Yersinia pestis
Int. J. Med. Microbiol.
295
87-98
2005
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669113
Chromy
Proteomic characterization of ...
Yersinia pestis
J. Bacteriol.
187
8172-8180
2005
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668759
Korhonen
-
Plasminogen activator of Yersi ...
Yersinia pestis
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
1
217-219
2004
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670148
Benedek
Intracellular signalling and c ...
Yersinia pestis
Microb. Pathog.
37
47-54
2004
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638926
Benedek
Mapping of possible laminin bi ...
Yersinia pestis
Adv. Exp. Med. Biol.
529
101-104
2003
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638923
Lahteenmaki
The Pla surface protease/adhes ...
Yersinia pestis
FEBS Lett.
504
69-72
2001
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1
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2
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5
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638924
Kukkonen
Protein regions important for ...
Yersinia pestis
Mol. Microbiol.
40
1097-1111
2001
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25
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5
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25
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1
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638925
Lahteenmaki
Bacterial plasminogen activato ...
Mammalia, Mycobacterium tuberculosis, Streptococcus uberis, Yersinia pestis
FEMS Microbiol. Rev.
25
531-552
2001
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4
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1
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638922
Kutyrev
Expression of the plague plasm ...
Yersinia pestis
Infect. Immun.
67
1359-1367
1999
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6
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638921
Lahteenmaki
Expression of plasminogen acti ...
Yersinia pestis
Infect. Immun.
66
5755-5762
1998
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638920
Kienle
-
Adhesive properties conferred ...
Yersinia pestis
J. Gen. Microbiol.
138
1679-1687
1992
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