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Literature summary for 3.4.23.45 extracted from

  • Kim, Y.T.; Downs, D.; Wu, S.; Dashti, A.; Pan, Y.; Zhai, P.; Wang, X.; Zhang, X.C.; Lin, X.
    Enzymic properties of recombinant BACE2 (2002), Eur. J. Biochem., 269, 5668-5677.
    View publication on PubMed
Search for more literature for 3.4.23.45

Cloned(Commentary)

Cloned (Comment) Organism
two variants of the pro-protease domain: pro-BACE2-T1 and pro-BACE2-T2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 70% inhibition at 1 mM Homo sapiens
Zn2+ 70% inhibition at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 gamma-secretase cleavage site of notch pH 6.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid-beta precursor protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two variants of the pro-protease domain: pro-BACE2-T1 and pro-BACE2-T2 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 pro-BACE2-T1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid-alpha pecursor protein + H2O
-
 Homo sapiens ?
-
 ?
amyloid-beta precursor protein + H2O
-
 Homo sapiens ?
-
 ?
gamma-secretase cleavage site of notch + H2O NCH-gamma Homo sapiens ?
-
 ?
insulin B chain + H2O only pro-BACE2-T1 Homo sapiens ?
-
 ?
kinetensin + H2O
-
 Homo sapiens ?
-
 ?
Mastoparan + H2O
-
 Homo sapiens ?
-
 ?
neuropetide + H2O Met-Lys-Arg-Ser-Arg-Gly-Pro-Ser-Pro-Arg-Arg Homo sapiens ?
-
 ?
preproenkephalin fragment 128-140 + H2O
-
 Homo sapiens ?
-
 ?
preproenkephalin fragment 129-138 + H2O ENK-1 Homo sapiens ?
-
 ?
Swedish amyloid-beta pecursor protein + H2O
-
 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 pro-BACE2-T1 with preproenkephalin fragment 129-138 as substrate Homo sapiens
9 10 pro-BACE2-T2 with gamma-secretase cleavage site of notch as substrate Homo sapiens
9.5
-
 pro-BACE2-T1 with gamma-secretase cleavage site of notch as substrate Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 stable, over 80% enzyme activity between pH 4 and pH 12 Homo sapiens