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show all sequences of 3.4.23.43

Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD

Aly, K.A.; Beebe, E.T.; Chan, C.H.; Goren, M.A.; Sepulveda, C.; Makino, S.; Fox, B.G.; Forest, K.T.; MicrobiologyOpen 2, 94-104 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
scaled-up synthesis of PilD, followed by solubilization in dodecyl-beta-D-maltoside and chromatography, leads to a pure enzyme that retains its known biochemical activities
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
PilD is a zinc-binding protein. Zinc is required for the N-terminal methylation of the mature pilin, but not for signal peptide cleavage
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
?
-
-
-
additional information
Pseudomonas aeruginosa ATCC 15692
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
?
-
-
-
prepilin PilA + H2O
Pseudomonas aeruginosa
-
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
?
prepilin PilA + H2O
Pseudomonas aeruginosa ATCC 15692
-
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
P22610
-
-
Pseudomonas aeruginosa ATCC 15692
P22610
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
734649
Pseudomonas aeruginosa
?
-
-
-
-
additional information
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
734649
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
prepilin PilA + H2O
-
734649
Pseudomonas aeruginosa
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
-
?
prepilin PilA + H2O
-
734649
Pseudomonas aeruginosa ATCC 15692
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
scaled-up synthesis of PilD, followed by solubilization in dodecyl-beta-D-maltoside and chromatography, leads to a pure enzyme that retains its known biochemical activities
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
PilD is a zinc-binding protein. Zinc is required for the N-terminal methylation of the mature pilin, but not for signal peptide cleavage
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
?
-
-
-
additional information
Pseudomonas aeruginosa ATCC 15692
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
?
-
-
-
prepilin PilA + H2O
Pseudomonas aeruginosa
-
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
?
prepilin PilA + H2O
Pseudomonas aeruginosa ATCC 15692
-
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
734649
Pseudomonas aeruginosa
?
-
-
-
-
additional information
enzyme additionally catalyzes S-adenosyl methionine-dependent methylation of the mature pilin
734649
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
prepilin PilA + H2O
-
734649
Pseudomonas aeruginosa
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
-
?
prepilin PilA + H2O
-
734649
Pseudomonas aeruginosa ATCC 15692
?
cosynthesis of PilD with its full-length substrate, PilA, leads to complete cleavage of the substrate signal peptides
-
-
?
Other publictions for EC 3.4.23.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Cell-free production of integr ...
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Bouhenni
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The role of Shewanella oneiden ...
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Electroanalysis
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Carter
The Pseudomonas aeruginosa pat ...
Pseudomonas aeruginosa
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Bernard
Organization and PprB-dependen ...
Pseudomonas aeruginosa
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191
1961-1973
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684559
Paranjpye
Role of type IV pilins in pers ...
Vibrio vulnificus
Appl. Environ. Microbiol.
73
5041-5044
2007
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669116
Szabo
Active-site residues in the ty ...
Saccharolobus solfataricus
J. Bacteriol.
188
1437-1443
2006
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1
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11
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669123
de Bentzmann
FppA, a novel Pseudomonas aeru ...
Pseudomonas aeruginosa
J. Bacteriol.
188
4851-4860
2006
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3
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Tomich
The TadV protein of Actinobaci ...
Aggregatibacter actinomycetemcomitans
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6899-6914
2006
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688467
Ng
Archaeal flagella, bacterial f ...
Escherichia coli, Methanococcus maripaludis, Methanococcus voltae, Pseudomonas aeruginosa, Saccharolobus solfataricus
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11
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2006
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670275
Akahane
Analysis of the pilU gene for ...
Escherichia coli
Mol. Genet. Genomics
273
350-359
2005
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43
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668803
Dupuy
-
Type IV prepilin peptidase ...
Bacillus subtilis, Dickeya chrysanthemi, Escherichia coli, Klebsiella pneumoniae, Legionella pneumoniae, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Vibrio cholerae, Vibrio vulnificus, Xanthomonas campestris
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1
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2004
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22
10
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LaPointe
The type 4 prepilin peptidases ...
Vibrio cholerae
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2000
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137314
Liles
The prepilin peptidase is requ ...
Legionella pneumophila
Mol. Microbiol.
31
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1999
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Identification of the Vibrio c ...
Vibrio cholerae
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29
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137311
Lory
Structure-function relationshi ...
Pseudomonas aeruginosa
Gene
192
117-121
1997
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137313
Bally
Protein secretion in Pseudomon ...
Pseudomonas aeruginosa
Mol. Microbiol.
6
1121-1131
1992
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137316
Pugsley
An enzyme with type IV prepili ...
Klebsiella oxytoca
Mol. Microbiol.
6
751-760
1992
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666764
Nunn
Components of the protein-excr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa mutant
Proc. Natl. Acad. Sci. USA
89
47-51
1992
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