BRENDA - Enzyme Database show
show all sequences of 3.4.23.43

Analysis of the pilU gene for the prepilin peptidase involved in the biogenesis of type IV pili encoded by plasmid R64

Akahane, K.; Sakai, D.; Furuya, N.; Komano, T.; Mol. Genet. Genomics 273, 350-359 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene pilU, expression of wild-type and mutant enzymes from plasmid R64 in strain DH5alpha and BL21(DE3)
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
A129V
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
A92V
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
C170R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
C200R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
D151N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
D91N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
E125G
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
F101L
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
F30Y
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
G95D
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
H76L
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
H76R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
I114N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
I23T
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
I24V
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
K138R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
K188R
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
K191E
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
K45R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L136P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L66S
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L81P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L93P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L96S
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L97I
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L97S
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
M110R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
M87K
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
additional information
effects of mutations on membrane topology of mutants, overview
Escherichia coli
N210D
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
Q86R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
S112P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
S82L
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
T102A
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
T128N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
V132D
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
V140D
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
W153R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
W161R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
W169G
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
W185R
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
W55R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
Y212C
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
gene pilU
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pilU, expression of wild-type and mutant enzymes from plasmid R64 in strain DH5alpha and BL21(DE3)
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A129V
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
A92V
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
C170R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
C200R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
D151N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
D91N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
E125G
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
F101L
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
F30Y
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
G95D
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
H76L
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
H76R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
I114N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
I23T
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
I24V
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
K138R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
K188R
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
K191E
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
K45R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L136P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L66S
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L81P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L93P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
L96S
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L97I
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
L97S
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
M110R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
M87K
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
additional information
effects of mutations on membrane topology of mutants, overview
Escherichia coli
N210D
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
Q86R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
S112P
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
S82L
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
T102A
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
T128N
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
V132D
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
V140D
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
W153R
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
W161R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
W169G
random mutagenesis, DNA sequence determination, reduced activity compared to the wild-type enzyme
Escherichia coli
W185R
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
W55R
random mutagenesis, DNA sequence determination, inactive mutant
Escherichia coli
Y212C
random mutagenesis, DNA sequence determination, highly reduced activity compared to the wild-type enzyme
Escherichia coli
Other publictions for EC 3.4.23.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734719
Linhartova
Accumulation of the Type IV pr ...
Synechocystis sp.
Mol. Microbiol.
93
1207-1223
2014
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1
1
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734649
Aly
Cell-free production of integr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692
MicrobiologyOpen
2
94-104
2013
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1
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1
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4
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6
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4
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717534
Bouhenni
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The role of Shewanella oneiden ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Electroanalysis
22
856-864
2010
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12
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6
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1
1
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717727
Carter
The Pseudomonas aeruginosa pat ...
Pseudomonas aeruginosa
J. Bacteriol.
192
3249-3258
2010
-
1
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3
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1
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1
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698610
Bernard
Organization and PprB-dependen ...
Pseudomonas aeruginosa
J. Bacteriol.
191
1961-1973
2009
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2
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684559
Paranjpye
Role of type IV pilins in pers ...
Vibrio vulnificus
Appl. Environ. Microbiol.
73
5041-5044
2007
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1
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5
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1
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669116
Szabo
Active-site residues in the ty ...
Saccharolobus solfataricus
J. Bacteriol.
188
1437-1443
2006
-
-
1
-
11
-
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-
1
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2
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3
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1
1
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11
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1
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3
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669123
de Bentzmann
FppA, a novel Pseudomonas aeru ...
Pseudomonas aeruginosa
J. Bacteriol.
188
4851-4860
2006
-
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1
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3
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1
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3
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1
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2
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669128
Tomich
The TadV protein of Actinobaci ...
Aggregatibacter actinomycetemcomitans
J. Bacteriol.
188
6899-6914
2006
-
-
1
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7
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688467
Ng
Archaeal flagella, bacterial f ...
Escherichia coli, Methanococcus maripaludis, Methanococcus voltae, Pseudomonas aeruginosa, Saccharolobus solfataricus
J. Mol. Microbiol. Biotechnol.
11
167-191
2006
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670275
Akahane
Analysis of the pilU gene for ...
Escherichia coli
Mol. Genet. Genomics
273
350-359
2005
-
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1
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43
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43
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668803
Dupuy
-
Type IV prepilin peptidase ...
Bacillus subtilis, Dickeya chrysanthemi, Escherichia coli, Klebsiella pneumoniae, Legionella pneumoniae, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Vibrio cholerae, Vibrio vulnificus, Xanthomonas campestris
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
204-208
2004
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1
10
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22
10
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10
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22
10
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1
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2
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137312
LaPointe
The type 4 prepilin peptidases ...
Vibrio cholerae
J. Biol. Chem.
275
1502-1510
2000
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1
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17
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2
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17
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1
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137314
Liles
The prepilin peptidase is requ ...
Legionella pneumophila
Mol. Microbiol.
31
959-970
1999
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1
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8
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137315
Marsh
Identification of the Vibrio c ...
Vibrio cholerae
Mol. Microbiol.
29
1481-1492
1998
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137311
Lory
Structure-function relationshi ...
Pseudomonas aeruginosa
Gene
192
117-121
1997
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4
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1
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137313
Bally
Protein secretion in Pseudomon ...
Pseudomonas aeruginosa
Mol. Microbiol.
6
1121-1131
1992
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1
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3
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137316
Pugsley
An enzyme with type IV prepili ...
Klebsiella oxytoca
Mol. Microbiol.
6
751-760
1992
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5
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666764
Nunn
Components of the protein-excr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa mutant
Proc. Natl. Acad. Sci. USA
89
47-51
1992
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1
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1
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4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-