Cloned (Comment) | Organism |
---|---|
gene tadV, DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3) | Aggregatibacter actinomycetemcomitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Flp1 prepilin + H2O | Aggregatibacter actinomycetemcomitans | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | Flp1 pilin + Flp1 prepilin leader peptide | - |
? | |
TadE prepseudopilin + H2O | Aggregatibacter actinomycetemcomitans | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | TadE pseudopilin + TadE prepseudopilin leader peptide | - |
? | |
TadF prepseudopilin + H2O | Aggregatibacter actinomycetemcomitans | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | TadF pseudopilin + TadF prepseudopilin leader peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aggregatibacter actinomycetemcomitans | - |
gene tadV of the tad locus | - |
Purification (Comment) | Organism |
---|---|
native TadV partially from Actinobacillus actinomycetemcomitans strains | Aggregatibacter actinomycetemcomitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Flp1 prepilin + H2O | - |
Aggregatibacter actinomycetemcomitans | Flp1 pilin + Flp1 prepilin leader peptide | - |
? | |
Flp1 prepilin + H2O | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | Aggregatibacter actinomycetemcomitans | Flp1 pilin + Flp1 prepilin leader peptide | - |
? | |
additional information | activity rate with different recombinant mutants of Flp1, TadE, and TadF constructed by site-directed mutagenesis and expressed in Escherichia coli, overview | Aggregatibacter actinomycetemcomitans | ? | - |
? | |
TadE prepseudopilin + H2O | - |
Aggregatibacter actinomycetemcomitans | TadE pseudopilin + TadE prepseudopilin leader peptide | - |
? | |
TadE prepseudopilin + H2O | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | Aggregatibacter actinomycetemcomitans | TadE pseudopilin + TadE prepseudopilin leader peptide | - |
? | |
TadF prepseudopilin + H2O | - |
Aggregatibacter actinomycetemcomitans | TadF pseudopilin + TadF prepseudopilin leader peptide | - |
? | |
TadF prepseudopilin + H2O | the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms | Aggregatibacter actinomycetemcomitans | TadF pseudopilin + TadF prepseudopilin leader peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TadV protein | - |
Aggregatibacter actinomycetemcomitans |