BRENDA - Enzyme Database show
show all sequences of 3.4.23.43

The TadV protein of Actinobacillus actinomycetemcomitans is a novel aspartic acid prepilin peptidase required for maturation of the Flp1 pilin and TadE and TadF pseudopilins

Tomich, M.; Fine, D.H.; Figurski, D.H.; J. Bacteriol. 188, 6899-6914 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene tadV, DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3)
Aggregatibacter actinomycetemcomitans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Flp1 prepilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
Flp1 pilin + Flp1 prepilin leader peptide
-
-
?
TadE prepseudopilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
?
TadF prepseudopilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aggregatibacter actinomycetemcomitans
-
gene tadV of the tad locus
-
Purification (Commentary)
Commentary
Organism
native TadV partially from Actinobacillus actinomycetemcomitans strains
Aggregatibacter actinomycetemcomitans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Flp1 prepilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
Flp1 pilin + Flp1 prepilin leader peptide
-
-
-
?
Flp1 prepilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
Flp1 pilin + Flp1 prepilin leader peptide
-
-
-
?
additional information
activity rate with different recombinant mutants of Flp1, TadE, and TadF constructed by site-directed mutagenesis and expressed in Escherichia coli, overview
669128
Aggregatibacter actinomycetemcomitans
?
-
-
-
-
TadE prepseudopilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
-
?
TadE prepseudopilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
-
?
TadF prepseudopilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
-
?
TadF prepseudopilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene tadV, DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3)
Aggregatibacter actinomycetemcomitans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Flp1 prepilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
Flp1 pilin + Flp1 prepilin leader peptide
-
-
?
TadE prepseudopilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
?
TadF prepseudopilin + H2O
Aggregatibacter actinomycetemcomitans
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native TadV partially from Actinobacillus actinomycetemcomitans strains
Aggregatibacter actinomycetemcomitans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Flp1 prepilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
Flp1 pilin + Flp1 prepilin leader peptide
-
-
-
?
Flp1 prepilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
Flp1 pilin + Flp1 prepilin leader peptide
-
-
-
?
additional information
activity rate with different recombinant mutants of Flp1, TadE, and TadF constructed by site-directed mutagenesis and expressed in Escherichia coli, overview
669128
Aggregatibacter actinomycetemcomitans
?
-
-
-
-
TadE prepseudopilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
-
?
TadE prepseudopilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
TadE pseudopilin + TadE prepseudopilin leader peptide
-
-
-
?
TadF prepseudopilin + H2O
-
669128
Aggregatibacter actinomycetemcomitans
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
-
?
TadF prepseudopilin + H2O
the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms
669128
Aggregatibacter actinomycetemcomitans
TadF pseudopilin + TadF prepseudopilin leader peptide
-
-
-
?
Other publictions for EC 3.4.23.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734719
Linhartova
Accumulation of the Type IV pr ...
Synechocystis sp.
Mol. Microbiol.
93
1207-1223
2014
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1
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734649
Aly
Cell-free production of integr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692
MicrobiologyOpen
2
94-104
2013
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6
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717534
Bouhenni
-
The role of Shewanella oneiden ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Electroanalysis
22
856-864
2010
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12
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6
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1
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717727
Carter
The Pseudomonas aeruginosa pat ...
Pseudomonas aeruginosa
J. Bacteriol.
192
3249-3258
2010
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1
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3
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1
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698610
Bernard
Organization and PprB-dependen ...
Pseudomonas aeruginosa
J. Bacteriol.
191
1961-1973
2009
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-
-
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2
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684559
Paranjpye
Role of type IV pilins in pers ...
Vibrio vulnificus
Appl. Environ. Microbiol.
73
5041-5044
2007
-
-
-
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1
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5
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1
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669116
Szabo
Active-site residues in the ty ...
Saccharolobus solfataricus
J. Bacteriol.
188
1437-1443
2006
-
-
1
-
11
-
-
-
1
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2
-
3
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1
1
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3
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11
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3
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-
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669123
de Bentzmann
FppA, a novel Pseudomonas aeru ...
Pseudomonas aeruginosa
J. Bacteriol.
188
4851-4860
2006
-
-
1
-
3
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1
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3
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-
-
2
-
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-
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669128
Tomich
The TadV protein of Actinobaci ...
Aggregatibacter actinomycetemcomitans
J. Bacteriol.
188
6899-6914
2006
-
-
1
-
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3
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2
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1
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7
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1
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7
-
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-
688467
Ng
Archaeal flagella, bacterial f ...
Escherichia coli, Methanococcus maripaludis, Methanococcus voltae, Pseudomonas aeruginosa, Saccharolobus solfataricus
J. Mol. Microbiol. Biotechnol.
11
167-191
2006
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670275
Akahane
Analysis of the pilU gene for ...
Escherichia coli
Mol. Genet. Genomics
273
350-359
2005
-
-
1
-
43
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2
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43
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668803
Dupuy
-
Type IV prepilin peptidase ...
Bacillus subtilis, Dickeya chrysanthemi, Escherichia coli, Klebsiella pneumoniae, Legionella pneumoniae, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Vibrio cholerae, Vibrio vulnificus, Xanthomonas campestris
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
204-208
2004
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10
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22
10
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1
10
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12
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22
10
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1
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2
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137312
LaPointe
The type 4 prepilin peptidases ...
Vibrio cholerae
J. Biol. Chem.
275
1502-1510
2000
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1
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17
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2
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17
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137314
Liles
The prepilin peptidase is requ ...
Legionella pneumophila
Mol. Microbiol.
31
959-970
1999
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1
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8
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137315
Marsh
Identification of the Vibrio c ...
Vibrio cholerae
Mol. Microbiol.
29
1481-1492
1998
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137311
Lory
Structure-function relationshi ...
Pseudomonas aeruginosa
Gene
192
117-121
1997
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4
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1
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137313
Bally
Protein secretion in Pseudomon ...
Pseudomonas aeruginosa
Mol. Microbiol.
6
1121-1131
1992
-
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1
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3
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2
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1
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2
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137316
Pugsley
An enzyme with type IV prepili ...
Klebsiella oxytoca
Mol. Microbiol.
6
751-760
1992
-
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-
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5
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1
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666764
Nunn
Components of the protein-excr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa mutant
Proc. Natl. Acad. Sci. USA
89
47-51
1992
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1
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1
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4
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1
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