BRENDA - Enzyme Database show
show all sequences of 3.4.23.42

Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius

Lin, X.L.; Tang, J.; J. Biol. Chem. 265, 1490-1495 (1990)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
;
Sulfolobus acidocaldarius
General Stability
General Stability
Organism
80C, 48 hours, pH 4.5
Sulfolobus acidocaldarius
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32651
-
1 * 32651, calculated from sequence
Sulfolobus acidocaldarius
46000
-
gel filtration; gel filtration
Sulfolobus acidocaldarius
51000
-
1 * 51000, SDS-PAGE; 1 * 51000, SDS-PAGE
Sulfolobus acidocaldarius
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfolobus acidocaldarius
P17118
precursor
-
Sulfolobus acidocaldarius
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sulfolobus acidocaldarius
side-chain modification
glycoprotein
Sulfolobus acidocaldarius
Purification (Commentary)
Commentary
Organism
;
Sulfolobus acidocaldarius
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hemoglobin + H2O
-
137307
Sulfolobus acidocaldarius
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 32651, calculated from sequence; 1 * 51000, SDS-PAGE; 1 * 51000, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
90
-
;
Sulfolobus acidocaldarius
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
60
100
60C: about 30% of maximal activity, 100C: about 70% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
stable
Sulfolobus acidocaldarius
80
-
pH 4.5, 48 h, stable
Sulfolobus acidocaldarius
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
-
;
Sulfolobus acidocaldarius
pH Range
pH Minimum
pH Maximum
Commentary
Organism
0.5
5
-
Sulfolobus acidocaldarius
1.3
3.7
pH 1.3: about 60% of maximal activity, pH 3.7: about 55% of maximal activity
Sulfolobus acidocaldarius
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Sulfolobus acidocaldarius
General Stability (protein specific)
General Stability
Organism
80C, 48 hours, pH 4.5
Sulfolobus acidocaldarius
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32651
-
1 * 32651, calculated from sequence
Sulfolobus acidocaldarius
46000
-
gel filtration
Sulfolobus acidocaldarius
51000
-
1 * 51000, SDS-PAGE
Sulfolobus acidocaldarius
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sulfolobus acidocaldarius
side-chain modification
glycoprotein
Sulfolobus acidocaldarius
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sulfolobus acidocaldarius
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hemoglobin + H2O
-
137307
Sulfolobus acidocaldarius
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 32651, calculated from sequence; 1 * 51000, SDS-PAGE
Sulfolobus acidocaldarius
monomer
1 * 51000, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
90
-
-
Sulfolobus acidocaldarius
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
60
100
60C: about 30% of maximal activity, 100C: about 70% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
stable
Sulfolobus acidocaldarius
80
-
pH 4.5, 48 h, stable
Sulfolobus acidocaldarius
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
-
-
Sulfolobus acidocaldarius
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
0.5
5
-
Sulfolobus acidocaldarius
1.3
3.7
pH 1.3: about 60% of maximal activity, pH 3.7: about 55% of maximal activity
Sulfolobus acidocaldarius
Other publictions for EC 3.4.23.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725163
Gogliettino
A new pepstatin-insensitive th ...
Saccharolobus solfataricus, Saccharolobus solfataricus DSM 1617
Int. J. Mol. Sci.
15
3204-3219
2014
-
-
-
-
-
-
1
-
-
-
-
-
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4
-
-
-
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-
-
-
-
9
-
1
-
1
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
-
9
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
726440
Cannio
Identification of a cell-bound ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Protein Pept. Lett.
17
78-85
2010
-
-
-
-
-
-
3
-
1
-
1
-
-
3
-
-
1
-
-
-
-
-
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
1
-
-
-
-
1
-
-
-
-
6
1
1
1
1
-
1
1
-
-
1
-
-
1
-
-
668807
Tang
-
Thermopsin ...
Sulfolobus acidocaldarius
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
225-227
2004
-
-
1
-
-
-
8
-
1
-
1
2
-
1
-
1
1
-
1
1
-
-
5
1
1
1
-
-
1
1
-
-
6
-
-
-
-
1
-
-
-
-
-
8
6
-
1
-
1
2
-
-
1
1
1
1
-
-
5
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
137308
Lin
Thermopsin ...
Sulfolobus acidocaldarius
Methods Enzymol.
248
156-168
1995
-
-
1
-
-
-
4
1
1
-
-
1
-
1
-
-
1
-
-
-
1
1
4
-
1
-
-
-
1
-
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-
-
-
-
-
-
1
-
-
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-
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4
-
1
1
-
-
1
-
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1
-
-
1
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
137309
Lin
Heterologous expression of the ...
Sulfolobus acidocaldarius
Enzyme Microb. Technol.
14
696-701
1992
-
-
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
5
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
5
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
137310
Lin
Thermopsin, a thermostable aci ...
Sulfolobus acidocaldarius
Adv. Exp. Med. Biol.
306
255-257
1991
-
-
1
-
-
-
4
-
-
-
-
-
-
2
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
137306
Fusek
Enzymic properties of thermops ...
Sulfolobus acidocaldarius
J. Biol. Chem.
265
1496-1501
1990
-
-
-
-
-
-
7
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
137307
Lin
Purification, characterization ...
Sulfolobus acidocaldarius
J. Biol. Chem.
265
1490-1495
1990
-
-
1
-
-
1
-
-
-
-
3
-
-
4
-
2
1
-
-
-
-
-
1
1
1
1
2
-
1
2
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
5
-
-
-
2
2
-
-
-
-
1
2
2
1
2
-
2
2
-
-
-
-
-
-
-
-