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Literature summary for 3.4.23.42 extracted from
- Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius (1990), J. Biol. Chem., 265, 1490-1495.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
General Stability
| General Stability | Organism |
|---|---|
| 80°C, 48 hours, pH 4.5 | Sulfolobus acidocaldarius |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32651 | - |
1 * 32651, calculated from sequence | Sulfolobus acidocaldarius |
| 46000 | - |
gel filtration | Sulfolobus acidocaldarius |
| 51000 | - |
1 * 51000, SDS-PAGE | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius | P17118 | precursor | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Sulfolobus acidocaldarius |
| side-chain modification | glycoprotein | Sulfolobus acidocaldarius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Sulfolobus acidocaldarius |
| monomer | 1 * 32651, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thpS | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 60°C: about 30% of maximal activity, 100°C: about 70% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable | Sulfolobus acidocaldarius |
| 80 | - |
pH 4.5, 48 h, stable | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 0.5 | 5 | - |
Sulfolobus acidocaldarius |
| 1.3 | 3.7 | pH 1.3: about 60% of maximal activity, pH 3.7: about 55% of maximal activity | Sulfolobus acidocaldarius |
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