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Literature summary for 3.4.23.40 extracted from

  • Almeida, C.M.; Manso, J.A.; Figueiredo, A.C.; Antunes, L.; Cruz, R.; Manadas, B.; Bur, D.; Pereira, P.J.B.; Faro, C.; Simoes, I.
    Functional and structural characterization of synthetic cardosin B-derived rennet (2017), Appl. Microbiol. Biotechnol., 101, 6951-6968 .
    View publication on PubMed
Search for more literature for 3.4.23.40

Cloned(Commentary)

Cloned (Comment) Organism
procardosin B with and without plant-specific insert domain is expressed in Kluyveromyces lactis strain GG799 Cynara cardunculus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by E-64, EDTA and Pefabloc Cynara cardunculus
pepstatin A
-
 Cynara cardunculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) native mature enzyme, at pH 4.0 and 37°C Cynara cardunculus
0.0087
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus
0.0094
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus Q9XFX4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein two putative glycosylation sites at N139 and N252 Cynara cardunculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) + H2O
-
 Cynara cardunculus ?
-
 ?
kappa-casein + H2O
-
 Cynara cardunculus para-kappa-casein + ?
-
 ?
additional information the enzyme shows a preference for accommodating large hydrophobic amino acids at position P1, such as phenylalanine, tyrosine, and leucine. In P1', the enzyme prefers rather small amino acids like valine, alanine, and serine Cynara cardunculus ?
-
 ?
skimmed milk + H2O
-
 Cynara cardunculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, recombinant procardosin B without plant-specific insert domain, SDS-PAGE Cynara cardunculus

Synonyms

Synonyms Comment Organism
cardosin B
-
 Cynara cardunculus
PCB proenzyme Cynara cardunculus
procardosin B proenzyme Cynara cardunculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Cynara cardunculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the enzyme has more than 50% activity in a temperature range of 30 to 60°C Cynara cardunculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.2
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus
24
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) native mature enzyme, at pH 4.0 and 37°C Cynara cardunculus
36.5
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Cynara cardunculus

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 the enzyme has more than 50% activity in a pH range of 3.0-5.5 Cynara cardunculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2000
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus
4200
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C Cynara cardunculus
17100
-
 ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) native mature enzyme, at pH 4.0 and 37°C Cynara cardunculus