BRENDA - Enzyme Database show
show all sequences of 3.4.23.40

A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance

Raimbault, A.K.; Zuily-Fodil, Y.; Soler, A.; Cruz de Carvalho, M.H.; J. Plant Physiol. 170, 1536-1540 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
food industry
gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties
Ananas comosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55230
-
x * 55230, calculated
Ananas comosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ananas comosus
K7P8F2
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
sequence has a signal peptide of 27 amino acids and a prosegment containing Lys36 which is important for the stability of the enzyme and for the correct alignmentof the active-center residues
Ananas comosus
Subunits
Subunits
Commentary
Organism
?
x * 55230, calculated
Ananas comosus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Ananas comosus
calculated
-
5.7
Application (protein specific)
Application
Commentary
Organism
food industry
gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties
Ananas comosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55230
-
x * 55230, calculated
Ananas comosus
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
sequence has a signal peptide of 27 amino acids and a prosegment containing Lys36 which is important for the stability of the enzyme and for the correct alignmentof the active-center residues
Ananas comosus
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55230, calculated
Ananas comosus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Ananas comosus
calculated
-
5.7
Expression
Organism
Commentary
Expression
Ananas comosus
gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties
additional information
Expression (protein specific)
Organism
Commentary
Expression
Ananas comosus
gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties
additional information
Other publictions for EC 3.4.23.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733194
Almeida
Engineering a cardosin B-deriv ...
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Appl. Microbiol. Biotechnol.
99
269-281
2015
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733901
Llorente
Use of artichoke (Cynara scoly ...
Cynara cardunculus var. scolymus
Food Chem.
159
55-63
2014
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734555
Raimbault
A novel aspartic acid protease ...
Ananas comosus
J. Plant Physiol.
170
1536-1540
2013
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734915
Pereira
Cardosin A contains two vacuol ...
Cynara cardunculus
Plant J.
76
87-100
2013
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4
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1
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669314
Castanheira
Activation, proteolytic proces ...
Cynara cardunculus
J. Biol. Chem.
280
13047-13054
2005
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1
-
2
1
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4
-
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3
-
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1
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-
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670515
Sidrach
Purification of cynarases from ...
Cynara cardunculus var. scolymus
Phytochemistry
66
41-49
2005
-
-
-
-
-
-
1
1
-
-
8
-
-
6
-
1
1
-
-
1
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1
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2
1
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2
-
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1
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2
1
1
2
-
3
-
-
-
-
-
-
649256
Prasad
Role of water molecules in the ...
Hordeum vulgare
Acta Crystallogr. Sect. D
D58
250-259
2002
-
-
-
1
-
-
-
-
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-
-
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1
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-
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-
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-
650630
Park
Autoproteolytic processing of ...
Helianthus annuus
Biosci. Biotechnol. Biochem.
65
702-705
2001
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
1
-
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1
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734130
Frazao
Crystal structure of cardosin ...
Cynara cardunculus
J. Biol. Chem.
274
27694-27701
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
1
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1
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-
30849
Belozersky
Aspartic proteinase from wheat ...
Triticum aestivum
Planta
177
321-326
1998
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
1
1
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-
1
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1
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-
1
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1
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2
-
1
-
-
-
-
-
-
-
733764
Verissimo
Purification, characterization ...
Cynara cardunculus
Eur. J. Biochem.
235
762-768
1996
-
-
-
-
-
-
2
4
-
-
3
-
-
3
-
-
-
-
-
-
1
-
4
1
-
-
1
4
1
1
-
-
2
-
-
-
-
-
-
-
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-
-
4
2
4
-
-
4
-
-
-
-
-
-
-
2
-
4
2
-
-
2
4
2
2
-
-
-
-
-
-
4
4
30844
Kervinen
Structure and possible functio ...
Brassica napus, Cannabis sativa, Cucumis sativus, Cucurbita maxima, Cynara cardunculus, Drosera peltata, Fagopyrum esculentum, Hordeum vulgare, Nelumbo nucifera, Nepenthes macfarlanei, Nicotiana tabacum, Oryza sativa, Pinus banksiana, Pinus sylvestris, Solanum lycopersicum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Triticum durum x Haynaldia villosa
Adv. Exp. Med. Biol.
362
241-254
1995
-
-
-
-
-
-
2
-
1
-
19
19
-
19
-
-
-
-
-
23
-
-
40
5
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
19
19
-
-
-
-
-
23
-
-
40
5
-
-
-
-
22
-
-
-
-
-
-
-
-
-
30845
Asakura
Rice aspartic proteinase, oryz ...
Oryza sativa
Eur. J. Biochem.
232
77-83
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
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1
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3
-
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-
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-
-
-
-
-
-
-
30847
Guruprasad
Comparative modelling of barle ...
Hordeum vulgare
FEBS Lett.
352
131-136
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
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-
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30848
Runeberg-Roos
The aspartic proteinase is a v ...
Hordeum vulgare
Plant Physiol.
105
321-329
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
3
-
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1
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-
-
-
-
-
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1
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3
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1
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-
-
-
-
-
-
-
-
-
-
30851
Törmäkangas
-
Tissue-specific localization o ...
Hordeum vulgare
Planta
195
116-125
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
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1
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-
-
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-
-
-
-
-
-
-
-
30853
Galleschi
-
Purification, characterization ...
Triticum aestivum
Plant Sci.
98
15-24
1994
-
-
-
-
-
-
1
1
-
-
2
-
-
1
-
-
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1
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1
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1
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1
1
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1
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30843
Kervinen
Hydrolytic specifity of barley ...
Hordeum vulgare
Phytochemistry
32
799-803
1993
-
-
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-
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-
-
-
-
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-
5
-
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3
-
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7
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3
-
-
7
-
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-
-
-
-
-
-
-
-
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-
-
-
30850
Sarkkinen
Aspartic proteinase from barle ...
Hordeum vulgare
Planta
186
317-323
1992
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-
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4
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4
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1
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1
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1
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4
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1
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1
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1
1
-
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-
-
-
-
-
30846
Runeberg-Roos
Primary structure of a barley- ...
Hordeum vulgare
Eur. J. Biochem.
202
1021-1027
1991
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-
1
-
-
-
-
-
-
-
-
-
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1
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30852
Galleschi
-
A rapid and sensitive method o ...
Triticum aestivum, Triticum durum x Haynaldia villosa
Plant Physiol. Biochem.
28
793-797
1990
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