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Literature summary for 3.4.23.40 extracted from

  • Almeida, C.M.; Gomes, D.; Faro, C.; Simoes, I.
    Engineering a cardosin B-derived rennet for sheep and goat cheese manufacture (2015), Appl. Microbiol. Biotechnol., 99, 269-281.
    View publication on PubMed

Application

Application Comment Organism
food industry use of recombinant enzyme for manufacturing sheep, goat, and cow cheeses result in a higher cheese yield for all three types of cheese when compared with synthetic chymosin Cynara cardunculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Kluyveromyces lactis Cynara cardunculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme Cynara cardunculus
52600
-
x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme Cynara cardunculus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus Q9XFX4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification optimum pH for proteolytic activation of recombinant enzyme is pH 4.0 Cynara cardunculus

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Cynara cardunculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O
-
Cynara cardunculus ? formation of two cleavage products of about 30 and 23 kDa ?
beta-casein + H2O
-
Cynara cardunculus ? formation of one single product of about 23 kDa ?
kappa-casein + H2O
-
Cynara cardunculus ? digestion of kappa-casein results in the appearance of a product of about 16 kDa ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme Cynara cardunculus

Synonyms

Synonyms Comment Organism
cardB
-
Cynara cardunculus
cardosin B
-
Cynara cardunculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cynara cardunculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 h, 80% residual activity Cynara cardunculus
60
-
2 h, 46% residual activity Cynara cardunculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Cynara cardunculus

Expression

Organism Comment Expression
Cynara cardunculus optimization of culture conditions for expression in Kluyveromyces lactis, best conditions are YPGal 4% for 120 h at 30°C. Subjecting the precursor form to a protein engineering approach followed by codon optimization leads to a clear improvement in the production of the protease, which also results in a reduction in expression time additional information