Application | Comment | Organism |
---|---|---|
food industry | use of recombinant enzyme for manufacturing sheep, goat, and cow cheeses result in a higher cheese yield for all three types of cheese when compared with synthetic chymosin | Cynara cardunculus |
Cloned (Comment) | Organism |
---|---|
expression in Kluyveromyces lactis | Cynara cardunculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme | Cynara cardunculus |
52600 | - |
x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme | Cynara cardunculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cynara cardunculus | Q9XFX4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | optimum pH for proteolytic activation of recombinant enzyme is pH 4.0 | Cynara cardunculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Cynara cardunculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | - |
Cynara cardunculus | ? | formation of two cleavage products of about 30 and 23 kDa | ? | |
beta-casein + H2O | - |
Cynara cardunculus | ? | formation of one single product of about 23 kDa | ? | |
kappa-casein + H2O | - |
Cynara cardunculus | ? | digestion of kappa-casein results in the appearance of a product of about 16 kDa | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme | Cynara cardunculus |
Synonyms | Comment | Organism |
---|---|---|
cardB | - |
Cynara cardunculus |
cardosin B | - |
Cynara cardunculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cynara cardunculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 h, 80% residual activity | Cynara cardunculus |
60 | - |
2 h, 46% residual activity | Cynara cardunculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Cynara cardunculus |
Organism | Comment | Expression |
---|---|---|
Cynara cardunculus | optimization of culture conditions for expression in Kluyveromyces lactis, best conditions are YPGal 4% for 120 h at 30°C. Subjecting the precursor form to a protein engineering approach followed by codon optimization leads to a clear improvement in the production of the protease, which also results in a reduction in expression time | additional information |