Protein Variants | Comment | Organism |
---|---|---|
D32A | active site mutant, inactive, no processing of proenzyme | Cynara cardunculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | - |
Cynara cardunculus | |
pepstatin A | - |
Cynara cardunculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | 37°C, pH 7.4 | Cynara cardunculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE | Cynara cardunculus |
26000 | - |
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE | Cynara cardunculus |
37000 | - |
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE | Cynara cardunculus |
47000 | - |
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE | Cynara cardunculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cynara cardunculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autocatalytic processing, optimal processing at pH 4.0 | Cynara cardunculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Cynara cardunculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu + H2O | - |
Cynara cardunculus | ? | - |
? | |
additional information | preference for phenylalanine, leucine or norleucine at P! site, for tyrosine or phenylalanine at P1 site | Cynara cardunculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE | Cynara cardunculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Cynara cardunculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65 | - |
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu | 37°C, pH 7.4 | Cynara cardunculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Cynara cardunculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | - |
Cynara cardunculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
pepstatin | 37°C, pH 7.4 | Cynara cardunculus |