Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.4 extracted from

  • Belenkaya, S.; Rudometov, A.; Shcherbakov, D.; Balabova, D.; Kriger, A.; Belov, A.; Koval, A.; Elchaninov, V.
    Biochemical properties of recombinant chymosin in alpaca (Vicugna pacos L.) (2018), Appl. Biochem. Microbiol., 54, 569-576 .
No PubMed abstract available
Search for more literature for 3.4.23.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bos taurus
expressed in Escherichia coli BL21(DE3) cells Vicugna pacos

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ Ca2+ inhibits milk-clotting activity (about 35% residual activity at 5 mM) Bos taurus
Ca2+ Ca2+ inhibits milk-clotting activity (about 50% residual activity at 5 mM) Vicugna pacos

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Vicugna pacos
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus
-
 Vicugna pacos

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bovine kappa-casein + H2O
-
 Bos taurus ?
-
 ?
bovine kappa-casein + H2O
-
 Vicugna pacos ?
-
 ?
milk + H2O
-
 Bos taurus ?
-
 ?
milk + H2O
-
 Vicugna pacos ?
-
 ?
additional information in the process of milk coagulation under the action of chymosin, alpha- and beta-caseins are not hydrolyzed Bos taurus ?
-
 ?
additional information in the process of milk coagulation under the action of chymosin, alpha- and beta-caseins are not hydrolyzed Vicugna pacos ?
-
 ?

Synonyms

Synonyms Comment Organism
rCH recombinant enzyme Bos taurus
rCH recombinant enzyme Vicugna pacos

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 60 the enzyme activity remains stable after 30 min at 35-60°C Vicugna pacos
35 55 the enzyme activity remains stable after 30 min at 35-55°C and drops to about 20% after 30 min at 60°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 pH optimum for milk-clotting activity Bos taurus
5.5
-
 pH optimum for milk-clotting activity Vicugna pacos

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6 100% activity at pH 5.5, about 20% activity at pH 6.0, the enzyme almost completely loses its activity at pH 7.0 Vicugna pacos
5.5 6 100% activity at pH 5.5, about 90% activity at pH 6.0, about 30% activity at pH 6.5, the enzyme almost completely loses its activity at pH 7.0 Bos taurus