Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | when subjected to a low pH, recombinant prochymosin is converted into mature and active chymosin | Camelus dromedarius |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Camelus dromedarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximum milk clotting activity is detected with 20-40 mM Ca2+ | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, active chymosin, SDS-PAGE | Camelus dromedarius |
42000 | - |
x * 42000, deglycosylated recombinant enzyme, SDS-PAGE | Camelus dromedarius |
45000 | - |
x * 45000, glycosylated recombinant enzyme, SDS-PAGE | Camelus dromedarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | Camelus dromedarius | - |
? | - |
? | |
additional information | Camelus dromedarius | does not cleave alpha-casein and beta-casein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | Q9GK11 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Camelus dromedarius |
Storage Stability | Organism |
---|---|
0-20°C, at pH 5.5, 8 h, no loss of activity | Camelus dromedarius |
40°C, at pH 5.5, 8 h, 37.5% residual activity | Camelus dromedarius |
50°C, at pH 5.5, complete loss of activity | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | - |
Camelus dromedarius | ? | - |
? | |
additional information | does not cleave alpha-casein and beta-casein | Camelus dromedarius | ? | - |
? | |
skim milk + H2O | - |
Camelus dromedarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, active chymosin, SDS-PAGE | Camelus dromedarius |
? | x * 42000, deglycosylated recombinant enzyme, SDS-PAGE | Camelus dromedarius |
? | x * 45000, glycosylated recombinant enzyme, SDS-PAGE | Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | optimum for milk clotting activity | Camelus dromedarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | relative activity of 50% is observed at temperatures of 35°C and 60°C. Chymosin activity completely ceases below 20°C or above 70°C | Camelus dromedarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | after 8 h of incubation, between 0 and 20°C the enzyme shows 100% activity, while at 30°C and 40°C 70% and 35% is retained, respectively. The enzyme is inactive after 8 h at 50°C | Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
optimum for milk clotting activity | Camelus dromedarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.1 | 6.7 | at pH 5.1, the relative clotting activity decreases to 50% and further declines to 17% and 10% at pH 5.6 and pH 6.7, respectively | Camelus dromedarius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6.5 | the recombinant enzyme is highly active and stable over a wide pH range (from 2.5 to 6.0) at 20°C for 8 h. Relative clotting activity declines when the recombinant chymosin is kept at a pH value above 6.0. No detectable clotting activity at pH 6.5 | Camelus dromedarius |