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Literature summary for 3.4.23.4 extracted from

  • Kumar, A.; Sharma, J.; Mohanty, A.K.; Grover, S.; Batish, V.K.
    Purification and characterization of milk clotting enzyme from goat (Capra hircus) (2006), Comp. Biochem. Physiol. B, 145, 108-113.
    View publication on PubMed

Application

Application Comment Organism
food industry used as milk coagulant in cheese preparation Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-casein + H2O Capra hircus cleaves a single bond between phenylalanine 105 and methionine 106 ?
-
?

Organism

Organism UniProt Comment Textmining
Capra hircus Q5VK60 synthetic construct of prochymosin mRNA; enzyme secretion is maximal during the first days after birth, enzyme level declines thereafter and chymosin in replaced by pepsin
-

Purification (Commentary)

Purification (Comment) Organism
resolves into three major peaks in DEAE chromatography Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
abdomen enzyme extracted from abdomasal tissue of 15 days old kid Capra hircus
-
stomach produced in the fourth stomach of milk fed ruminants as inactive precursor prochymosin Capra hircus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-casein + H2O
-
Capra hircus ?
-
?
kappa-casein + H2O cleaves a single bond between phenylalanine 105 and methionine 106 Capra hircus ?
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Capra hircus

Synonyms

Synonyms Comment Organism
More acid protease, major component of rennet (milk clotting enzyme) Capra hircus
prochymosin inactive precursor Capra hircus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
the substrate temperature was found to influence milk clotting activity, optimum substrate temperature is 65°C Capra hircus
30
-
milk clotting activity Capra hircus
55
-
proteolytic activity Capra hircus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
the substrate temperature was found to influence milk clotting activity, sharp optimum at 65°C with complete loss of activity at 75°C, less than 20% of activity at 30°C Capra hircus
30 60 milk clotting activity declines moderately up to 55°C (80% of maximum activity) and than drops to 40% of activity at 60°C proteolytic activity at 30°C and 60°C approximately 80% of maximum activity Capra hircus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
stable up to 55°C, more thermostable than cattle chymosin and equally stable as buffalo chymosin Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Capra hircus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 sharp decline of activity up to pH 5.8, moderate decline at higher pH with complete loss of activity at pH 8 Capra hircus