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Literature summary for 3.4.23.4 extracted from

  • Pitts, J.E.; Quinn, D.; Uusitalo, J.; Penttilae, M.
    Protein engineering of chymosin and expression in Trichoderma reesei (1991), Food Biotechnol., 19, 663-666.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
point mutants expressed in Escherichia coli and Trichoderma reesei Bos taurus

Protein Variants

Protein Variants Comment Organism
D304A/T218A double mutant, substrate-specificity-pocket variant Bos taurus
Q288K
-
Bos taurus
T218A pH optima shift Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-casein + H2O Bos taurus cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-casein + H2O cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 Bos taurus ?
-
?
Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu-OH + H2O
-
Bos taurus Lys-Pro-Ala-Glu-Phe + Phe(NO2)-Ala-Leu
-
?