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Literature summary for 3.4.23.4 extracted from
- Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin (1997), Protein Eng., 10, 991-997.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| point mutants expressed in Escherichia coli and Trichoderma reesei | Bos taurus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| point mutants | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A115T | reduction of Km compared to wild-type | Bos taurus |
| G243D | increase of Km compared to wild-type | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
kappa-casein | point mutant G343D | Bos taurus | |
| 0.333 | - |
Leu-Ser-Phe(NO2)-Nle-Ala-Leu-O-methyl ester | point mutant G243D | Bos taurus |  |
| 1.11 | - |
Leu-Ser-Phe(NO2)-Nle-Ala-Leu-Ome | point mutant A115T | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-casein + H2O | Bos taurus | cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| His-Pro-His-Pro-His-Leu-Ser-Phe-Phe(NO2)-Ala-Ile-Pro-Pro-Lys-Lys + H2O | - |
Bos taurus | ? | - |
? | |
| kappa-casein + H2O | cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 | Bos taurus | p-kappa-casein + glycomacropeptide | - |
? | |
| kappa-casein + H2O | cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 | Bos taurus | ? | - |
? | |
| Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe + H2O | - |
Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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