Activating Compound | Comment | Organism | Structure |
---|---|---|---|
detergent | required for activity, e.g. Triton X-100 or Nikkol, but not lauryl sarcoside or octylglucoside | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Staphylococcus aureus |
DNA and amino acid sequence determination and analysis | Pseudomonas fluorescens |
DNA and amino acid sequence determination and analysis | Klebsiella aerogenes |
enzyme overexpression in Escherichia coli confers high resistance to globomycin | Myxococcus xanthus |
gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Globomycin | - |
Bacillus subtilis | |
Globomycin | uncompetitive | Escherichia coli | |
Globomycin | - |
Klebsiella aerogenes | |
Globomycin | - |
Myxococcus xanthus | |
Globomycin | - |
Pseudomonas fluorescens | |
Globomycin | - |
Staphylococcus aureus | |
HgCl2 | - |
Escherichia coli | |
lauroyl sarcoside | - |
Escherichia coli | |
octylglucoside | - |
Escherichia coli | |
tosyl arginyl methyl ester | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Staphylococcus aureus | - |
- |
inner membrane | - |
Bacillus subtilis | - |
- |
inner membrane | - |
Pseudomonas fluorescens | - |
- |
inner membrane | - |
Klebsiella aerogenes | - |
- |
inner membrane | - |
Myxococcus xanthus | - |
- |
inner membrane | transmembrane protein with a large and a small loop at the periplasmic side | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Staphylococcus aureus | |
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Bacillus subtilis | |
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Escherichia coli | |
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Pseudomonas fluorescens | |
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Klebsiella aerogenes | |
additional information | no requirement for metal ions, thiol reagents, and phospholipids | Myxococcus xanthus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
18144 | - |
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacterial lipoprotein + H2O | Staphylococcus aureus | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
bacterial lipoprotein + H2O | Bacillus subtilis | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
bacterial lipoprotein + H2O | Escherichia coli | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
bacterial lipoprotein + H2O | Pseudomonas fluorescens | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
bacterial lipoprotein + H2O | Klebsiella aerogenes | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
bacterial lipoprotein + H2O | Myxococcus xanthus | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
gene lsp | - |
Klebsiella aerogenes | - |
- |
- |
Myxococcus xanthus | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme | Escherichia coli |
proteolytic modification | N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme | Escherichia coli |
Purification (Comment) | Organism |
---|---|
native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65°C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Staphylococcus aureus | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Bacillus subtilis | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Escherichia coli | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Pseudomonas fluorescens | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Klebsiella aerogenes | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Myxococcus xanthus | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Staphylococcus aureus | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Bacillus subtilis | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Escherichia coli | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Pseudomonas fluorescens | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Klebsiella aerogenes | ? | - |
? | |
bacterial lipoprotein + H2O | cleavage of the signal peptide | Myxococcus xanthus | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Staphylococcus aureus | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Bacillus subtilis | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Escherichia coli | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Pseudomonas fluorescens | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Klebsiella aerogenes | ? | - |
? | |
Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Myxococcus xanthus | ? | - |
? | |
additional information | active site catalytic residues are Asp102 and Asp129 | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
More | primary structure | Staphylococcus aureus |
More | primary structure | Escherichia coli |
More | primary structure | Pseudomonas fluorescens |
More | primary structure | Klebsiella aerogenes |
Synonyms | Comment | Organism |
---|---|---|
lipoprotein-specific signal peptidase | - |
Escherichia coli |
More | the enzyme belongs to the A8 peptidase family | Staphylococcus aureus |
More | the enzyme belongs to the A8 peptidase family | Bacillus subtilis |
More | the enzyme belongs to the A8 peptidase family | Escherichia coli |
More | the enzyme belongs to the A8 peptidase family | Pseudomonas fluorescens |
More | the enzyme belongs to the A8 peptidase family | Klebsiella aerogenes |
More | the enzyme belongs to the A8 peptidase family | Myxococcus xanthus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified enzyme, stable for a short time | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
neutral pH optimum | Staphylococcus aureus |
additional information | - |
neutral pH optimum | Bacillus subtilis |
additional information | - |
neutral pH optimum | Pseudomonas fluorescens |
additional information | - |
neutral pH optimum | Klebsiella aerogenes |
additional information | - |
neutral pH optimum | Myxococcus xanthus |
6 | - |
purified enzyme | Escherichia coli |
7.9 | - |
crude enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for cofactors | Staphylococcus aureus | |
additional information | no requirement for cofactors | Bacillus subtilis | |
additional information | no requirement for cofactors | Escherichia coli | |
additional information | no requirement for cofactors | Pseudomonas fluorescens | |
additional information | no requirement for cofactors | Klebsiella aerogenes | |
additional information | no requirement for cofactors | Myxococcus xanthus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000036 | - |
Globomycin | - |
Escherichia coli |