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Literature summary for 3.4.23.36 extracted from

  • Sankaran, K.
    Signal peptidase II (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 201-204.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
detergent required for activity, e.g. Triton X-100 or Nikkol, but not lauryl sarcoside or octylglucoside Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Staphylococcus aureus
DNA and amino acid sequence determination and analysis Pseudomonas fluorescens
DNA and amino acid sequence determination and analysis Klebsiella aerogenes
enzyme overexpression in Escherichia coli confers high resistance to globomycin Myxococcus xanthus
gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Globomycin
-
Bacillus subtilis
Globomycin uncompetitive Escherichia coli
Globomycin
-
Klebsiella aerogenes
Globomycin
-
Myxococcus xanthus
Globomycin
-
Pseudomonas fluorescens
Globomycin
-
Staphylococcus aureus
HgCl2
-
Escherichia coli
lauroyl sarcoside
-
Escherichia coli
octylglucoside
-
Escherichia coli
tosyl arginyl methyl ester
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
Staphylococcus aureus
-
-
inner membrane
-
Bacillus subtilis
-
-
inner membrane
-
Pseudomonas fluorescens
-
-
inner membrane
-
Klebsiella aerogenes
-
-
inner membrane
-
Myxococcus xanthus
-
-
inner membrane transmembrane protein with a large and a small loop at the periplasmic side Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions, thiol reagents, and phospholipids Staphylococcus aureus
additional information no requirement for metal ions, thiol reagents, and phospholipids Bacillus subtilis
additional information no requirement for metal ions, thiol reagents, and phospholipids Escherichia coli
additional information no requirement for metal ions, thiol reagents, and phospholipids Pseudomonas fluorescens
additional information no requirement for metal ions, thiol reagents, and phospholipids Klebsiella aerogenes
additional information no requirement for metal ions, thiol reagents, and phospholipids Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE Escherichia coli
18144
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacterial lipoprotein + H2O Staphylococcus aureus cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?
bacterial lipoprotein + H2O Bacillus subtilis cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?
bacterial lipoprotein + H2O Escherichia coli cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?
bacterial lipoprotein + H2O Pseudomonas fluorescens cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?
bacterial lipoprotein + H2O Klebsiella aerogenes cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?
bacterial lipoprotein + H2O Myxococcus xanthus cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
gene lsp
-
Klebsiella aerogenes
-
-
-
Myxococcus xanthus
-
-
-
Pseudomonas fluorescens
-
-
-
Staphylococcus aureus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme Escherichia coli
proteolytic modification N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65°C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Staphylococcus aureus ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Bacillus subtilis ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Escherichia coli ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Pseudomonas fluorescens ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Klebsiella aerogenes ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis Myxococcus xanthus ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Staphylococcus aureus ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Bacillus subtilis ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Escherichia coli ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Pseudomonas fluorescens ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Klebsiella aerogenes ?
-
?
bacterial lipoprotein + H2O cleavage of the signal peptide Myxococcus xanthus ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Staphylococcus aureus ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Bacillus subtilis ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Escherichia coli ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Pseudomonas fluorescens ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Klebsiella aerogenes ?
-
?
Braun's polylipoprotein + H2O cleavage of the signal peptide, cleavage site specificity, overview Myxococcus xanthus ?
-
?
additional information active site catalytic residues are Asp102 and Asp129 Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
? x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE Escherichia coli
More primary structure Staphylococcus aureus
More primary structure Escherichia coli
More primary structure Pseudomonas fluorescens
More primary structure Klebsiella aerogenes

Synonyms

Synonyms Comment Organism
lipoprotein-specific signal peptidase
-
Escherichia coli
More the enzyme belongs to the A8 peptidase family Staphylococcus aureus
More the enzyme belongs to the A8 peptidase family Bacillus subtilis
More the enzyme belongs to the A8 peptidase family Escherichia coli
More the enzyme belongs to the A8 peptidase family Pseudomonas fluorescens
More the enzyme belongs to the A8 peptidase family Klebsiella aerogenes
More the enzyme belongs to the A8 peptidase family Myxococcus xanthus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
purified enzyme, stable for a short time Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
neutral pH optimum Staphylococcus aureus
additional information
-
neutral pH optimum Bacillus subtilis
additional information
-
neutral pH optimum Pseudomonas fluorescens
additional information
-
neutral pH optimum Klebsiella aerogenes
additional information
-
neutral pH optimum Myxococcus xanthus
6
-
purified enzyme Escherichia coli
7.9
-
crude enzyme Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for cofactors Staphylococcus aureus
additional information no requirement for cofactors Bacillus subtilis
additional information no requirement for cofactors Escherichia coli
additional information no requirement for cofactors Pseudomonas fluorescens
additional information no requirement for cofactors Klebsiella aerogenes
additional information no requirement for cofactors Myxococcus xanthus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000036
-
Globomycin
-
Escherichia coli