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Literature summary for 3.4.23.35 extracted from
- Properties of Barrier, a novel Saccharomyces cerevisiae acid protease (1993), Biochimie, 75, 467-472.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Leu-Gln-Leu-Lys, Leu-Gln-Leu-Lys-Pro, Trp-Leu-Gln-Leu, His-Trp-Leu-Gln-Leu, Trp-Leu-Gln-Leu-Lys, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro, Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-lactone, Saccharomyces kluyveri alpha factor, tosyl-L-Lys chloromethylketone, 1,2-epoxy-3-(4-nitrophenoxy)propane, diazoacetyl-DL-norleucine ester, pepstatin; Trp-Leu-Gln-Leu-Lys-Pro | Saccharomyces cerevisiae | |
| tosyl-Arg methyl ester | at pH 6 and above, not at low pH values | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
alpha factor | pH 5.5, 0.1 M acetate buffer | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha Factor + H2O | Saccharomyces cerevisiae | i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, involved in physiological changes preceding Saccharomyces cerevisiae cell fusion | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast mutant strains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from Saccharomyces cerevisiae XP635 cells transformed with pZV24 | Saccharomyces cerevisiae |
| the heavy glycosylation allows enzyme precipitation with ethanol | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme precipitated by cold ethanol and resuspended in water | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha Factor + H2O | strict substrate specificity | Saccharomyces cerevisiae | Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr | - |
? | |
| alpha Factor + H2O | i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, involved in physiological changes preceding Saccharomyces cerevisiae cell fusion | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | Trp-Leu-Gln-Leu-Lys-Pro | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | Leu-Gln-Leu-Lys, Leu-Gln-Leu-Lys-Pro, Trp-Leu-Gln-Leu, His-Trp-Leu-Gln-Leu, Trp-Leu-Gln-Leu-Lys, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro, Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-lactone, Saccharomyces kluyveri alpha factor, tosyl-L-Lys chloromethylketone, tosyl-L-Arg methyl ester, 1,2-epoxy-3-(4-nitrophenoxy)propane, diazoacetyl-DL-norleucine ester, pepstatin | Saccharomyces cerevisiae | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.3 | dependent on buffer species, maximal activity in 0.1 M phosphate buffer | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 6.5 | about half-maximal activity at pH 2.5 and 6.5 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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