Cloned (Comment) | Organism |
---|---|
heterologously expressed in human embryonic kidney 293T cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D283A | site-directed mutagenesis | Rattus norvegicus |
D283E | site-directed mutagenesis | Rattus norvegicus |
D98A | site-directed mutagenesis | Rattus norvegicus |
D98A/D283A | double mutant, site-directed mutagenesis | Rattus norvegicus |
D98E | site-directed mutagenesis | Rattus norvegicus |
D98E/D283E | double mutant, site-directed mutagenesis | Rattus norvegicus |
T284S | site-directed mutagenesis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ascaris pepsin inhibitor | - |
Rattus norvegicus | |
pepstatin A | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.27 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, wild-type | Rattus norvegicus | |
1.31 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, mutant D98E | Rattus norvegicus | |
1.91 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, mutant T284S | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Rattus norvegicus | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
wild-type and T284S and D98E mutant proteins | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 + H2O | fluorogenic synthetic substrate | Rattus norvegicus | ? | - |
? | |
Hemoglobin + H2O | denatured | Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.55 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, mutant D98E | Rattus norvegicus | |
6.4 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, mutant T284S | Rattus norvegicus | |
11.9 | - |
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 | pH 3.5, 40°C, wild-type | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000582 | - |
pepstatin A | pH 3.5, 40°C, wild-type | Rattus norvegicus | |
0.00000606 | - |
pepstatin A | pH 3.5, 40°C, mutant D98E | Rattus norvegicus | |
0.00000648 | - |
pepstatin A | pH 3.5, 40°C, mutant T284S | Rattus norvegicus | |
0.00001834 | - |
Ascaris pepsin inhibitor | pH 3.5, 40°C, wild-type | Rattus norvegicus | |
0.00001913 | - |
Ascaris pepsin ihibitor | pH 3.5, 40°C, mutant D98E | Rattus norvegicus | |
0.00001953 | - |
Ascaris pepsin inhibitor | pH 3.5, 40°C, mutant T284S | Rattus norvegicus |