General Stability | Organism |
---|---|
Freezing inactivates, 50% glycerol stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | C4A mutant cathepsin E, pH 3.1 | Homo sapiens | |
0.045 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | C4A mutant cathepsin E, pH 3.1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
monomeric form | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Homo sapiens | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide | Homo sapiens | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the temperature stability of the mutant monomeric form in alkaline solution is markedly reduced | Homo sapiens |
25 | - |
and below, stable, t1/2: more than 3 h at pH 9.5 | Homo sapiens |
37 | - |
t1/2: about 16 h at pH 8.5, t1/2: 40 min at pH 9.5, t1/2: 3 min at pH 10.5 | Homo sapiens |
45 | - |
t1/2: 40 min at pH 8.5 | Homo sapiens |
55 | - |
t1/2: 35 min (native enzyme) or 1.7 min (recombinant enzyme) at pH 7.5, t1/2: less than 5 min at pH 8.5 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | Cys4-Ala mutant cathepsin E, pH 3.1 | Homo sapiens | |
82 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | Cys4-Ala mutant cathepsin E, pH 3.1 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pH-stability of the mutant monomeric form in alkaline solution is markedly reduced | Homo sapiens |