Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the catalytically inactive proenzyme procathepsin E is rapidly converted to the active enzyme cathepsin E after brief treatment at pH 4 by autoproteolytic cleavage at Met36-Ile37 and Phe39-Thr40 to form two mature isozymic forms | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2 | i.e. L-363,564, hemoglobin as substrate | Homo sapiens | |
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu | hemoglobin as substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent Km-values of hemoglobin hydrolysis | Homo sapiens | |
0.04 | - |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu | Homo sapiens | |
0.13 | - |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | non-lysosomal proteinase | Homo sapiens | 5622 | - |
membrane | - |
Homo sapiens | 16020 | - |
soluble | activation by solubilization from membrane | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
human, SDS-PAGE, non-reducing conditions | Homo sapiens |
90000 | - |
human | Homo sapiens |
90000 | - |
procathepsin E, SDS-PAGE, non-reducing conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzymes: N-glycosylated with high-mannose type oligosaccharide chain | Homo sapiens |
glycoprotein | rat spleen enzyme | Homo sapiens |
glycoprotein | human and rat erythrocyte enzyme: endo-beta-N-acetylglucosaminidase H-resistant complex or hybrid-type oligosaccharide chain | Homo sapiens |
Purification (Comment) | Organism |
---|---|
immunoaffinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | at pH 5.5 | Homo sapiens | hydrolyzed casein | - |
? | |
Hemoglobin + H2O | - |
Homo sapiens | Hydrolyzed hemoglobin | - |
? | |
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Homo sapiens | Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu | - |
? | |
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O | synthetic chromogenic peptide | Homo sapiens | Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE, reducing conditions | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Homo sapiens | |
ATP | hemoglobin as substrate | Homo sapiens | |
ATP | 6.25 mM | Homo sapiens | |
ATP | no activation of casein hydrolysis at pH 5.5 | Homo sapiens |