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Literature summary for 3.4.23.25 extracted from
- The structure and function of Saccharomyces cerevisiae proteinase A (2007), Yeast, 24, 467-480.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(p-nitrophenoxy) propane | - |
Saccharomyces cerevisiae |  |
| CP-108,420 | - |
Saccharomyces cerevisiae | |
| CP-72,647 | - |
Saccharomyces cerevisiae | |
| CP-81,198 | - |
Saccharomyces cerevisiae | |
| CP-81,282 | - |
Saccharomyces cerevisiae | |
| Diazoacetyl-D,L-norleucine methyl ester | - |
Saccharomyces cerevisiae | |
| IA3 | peptide inhibitor synthesized by Saccharomyces cerevisae, a synthetic peptide containing residues 2-34 of IA3 exhibits comparable potency to natural IA3 | Saccharomyces cerevisiae | |
| PD-129,541 | - |
Saccharomyces cerevisiae | |
| PD-133,450 | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | synthesized as an inactive precursor (zymogen), termed preproPrA, which transits to the endoplasmic reticulum where the protein is glycosylated and a hydrophobic signal peptide of the 22 amino acid is removed, the protein is then transported to the Golgi complex, where the carbohydrate side chains are modified by mannosyltransferases, the resulting 52 kDa proPrA is transported through the endosome to the vacuole, where a 54-amino acid propeptide is removed, yielding the mature 42 kDa proteinase | Saccharomyces cerevisiae | - |
- |
| vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | essential to the activities of other yeast vacuolar hydrolases, including proteinase B, aminopeptidase I, and carboxypeptidase Y | ? | - |
? | |
| proproteinase A + H2O | Saccharomyces cerevisiae | autoactivation of the enzyme yields a functional protein cleaved after Ser68, autoactivation can occur in rare situations when PrB is unable to activate PrA | pseudo-proteinaseA + peptide | Ser68 is the N-terminal amino acid | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P07267 | wild type and proteinase B deficiency strain, in wild-type strains, proPrA is activated to mature PrA with a molecular weight of 42000 Da, whereas in PrB-deficient strains PrA maturation is delayed and a mature pseudo-form of PrA with a molecular weight of 43000 Da is detected | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycosylation at Asn67 and Asn266 | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-/-Val-Tyr bond in a synthetic substrate. Does not act on esters of Tyr or Arg | description of mechanism, two catalytically active aspartic acid residues, Asp32 and Asp215 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| A-L-S-A-F-(4NO2)F-R-L + H2O | very effective substrate | Saccharomyces cerevisiae | ? | - |
? | |
| A-P-A-K-F-(4NO2)F-R-L + H2O | very effective substrate | Saccharomyces cerevisiae | ? | - |
? | |
| K-L-A-K-F-(4NO2)F-R-L + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| K-P-A-A-F-(4NO2)F-R-L + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| K-P-A-K-F-(4NO2)F-R-L + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| K-P-S-K-F-(4NO2)F-R-L + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | essential to the activities of other yeast vacuolar hydrolases, including proteinase B, aminopeptidase I, and carboxypeptidase Y | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | preference for hydrophobic residues with Phe, Leu or Glu at the P1 position and Phe, Ile, Leu or Ala at P1 | Saccharomyces cerevisiae | ? | - |
? | |
| proproteinase A + H2O | autoactivation of the enzyme yields a functional protein cleaved after Ser68, autoactivation can occur in rare situations when PrB is unable to activate PrA | Saccharomyces cerevisiae | pseudo-proteinaseA + peptide | Ser68 is the N-terminal amino acid | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | synthesized as inactive precursor of 405 residues with an apparent molecular weight of 52000 Da, mature nonglycosylated form of PrA has a molecular weight of 35800 Da, mature glycosylated form of PrA has a molecular weight of 41500 Da | Saccharomyces cerevisiae |
| More | in PrB-deficient strains PrA maturation is delayed and a mature pseudo-form of PrA with a molecular weight of 43000 Da is detected | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pepsin-like aspartic proteinase | - |
Saccharomyces cerevisiae |
| PRA | - |
Saccharomyces cerevisiae |
| preproPrA | inactive non-glycosylated precursor | Saccharomyces cerevisiae |
| proPrA | inactive glycosylated precursor | Saccharomyces cerevisiae |
| Proteinase A | - |
Saccharomyces cerevisiae |
| proteinase A precursor | inactive, maturation into active enzyme required proteinase B activity, 405 residues long precursor and 329 residues for the mature protein (77-405) | Saccharomyces cerevisiae |
| pseudo-proteinase A | active form of the enzyme resulting from autoactivation | Saccharomyces cerevisiae |
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