BRENDA - Enzyme Database show
show all sequences of 3.4.23.25

The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix

Li, M.; Phylip, L.H.; Lees, W.E.; Winther, J.R.; Dunn, B.M.; Wlodawer, A.; Kay, J.; Gustchina, A.; Nat. Struct. Biol. 7, 113-117 (2000)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
bound to inhibitor protein IA3, vapor diffusion, space group P6(2)22, unit cell dimensions a = b = 192.66 A, c = 52.09 A
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
protein IA3
Saccharomyces cerevisiae protein
Saccharomyces cerevisiae
protein IA3 mutant 2-34
-
Saccharomyces cerevisiae
protein IA3 mutant M31-M32
-
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
yeast
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Lys-Pro-Ile-Glu-Phe-(NO2)Phe-Arg-Leu + H2O
-
653309
Saccharomyces cerevisiae
Lys-Pro-Ile-Glu-Phe + (NO2)Phe-Arg-Leu
-
653309
Saccharomyces cerevisiae
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000009
-
protein IA3 mutant M31-M32
pH 4.7, 37°C
Saccharomyces cerevisiae
0.0000011
-
protein IA3
pH 4.7, 37°C
Saccharomyces cerevisiae
0.000003
-
protein IA3 mutant 2-34
pH 4.7, 37°C
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
bound to inhibitor protein IA3, vapor diffusion, space group P6(2)22, unit cell dimensions a = b = 192.66 A, c = 52.09 A
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
protein IA3
Saccharomyces cerevisiae protein
Saccharomyces cerevisiae
protein IA3 mutant 2-34
-
Saccharomyces cerevisiae
protein IA3 mutant M31-M32
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000009
-
protein IA3 mutant M31-M32
pH 4.7, 37°C
Saccharomyces cerevisiae
0.0000011
-
protein IA3
pH 4.7, 37°C
Saccharomyces cerevisiae
0.000003
-
protein IA3 mutant 2-34
pH 4.7, 37°C
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Lys-Pro-Ile-Glu-Phe-(NO2)Phe-Arg-Leu + H2O
-
653309
Saccharomyces cerevisiae
Lys-Pro-Ile-Glu-Phe + (NO2)Phe-Arg-Leu
-
653309
Saccharomyces cerevisiae
?
Other publictions for EC 3.4.23.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717794
Spedale
Identification of Pep4p as the ...
Saccharomyces cerevisiae
J. Biol. Chem.
285
22793-22799
2010
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1
1
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718128
Pereira
Mitochondrial degradation in a ...
Saccharomyces cerevisiae
Mol. Microbiol.
76
1398-1410
2010
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1
1
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683560
Bolumar
Purification and characterisat ...
Debaryomyces hansenii, Debaryomyces hansenii CECT12487
Int. J. Food Microbiol.
124
135-141
2008
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1
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698459
Narayanan
Kinetics of folding and bindin ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
130
11477-11485
2008
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683009
Parr
The structure and function of ...
Saccharomyces cerevisiae
Yeast
24
467-480
2007
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9
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2
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2
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2
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1
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9
2
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2
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687670
Winterburn
Key features determining the s ...
Saccharomyces cerevisiae
J. Biol. Chem.
282
6508-6516
2007
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3
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1
4
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5
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3
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683085
Kato
Analysis of a processing syste ...
Saccharomyces cerevisiae
Appl. Microbiol. Biotechnol.
72
1229-1237
2006
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1
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1
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6
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2
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6
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6
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686781
Guilloux-Benatier
Effects of yeast proteolytic a ...
Saccharomyces cerevisiae
FEMS Microbiol. Lett.
263
183-188
2006
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1
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688855
Marques
The Pep4p vacuolar proteinase ...
Saccharomyces cerevisiae
Microbiology
152
3595-3605
2006
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2
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671016
Bae
Cloning and characterization o ...
Ogataea angusta
Yeast
22
13-19
2005
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1
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1
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667602
Green
IA3, an aspartic proteinase in ...
Saccharomyces cerevisiae
Biochemistry
43
4071-4081
2004
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649600
Gustchina
An unusual orientation for Tyr ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
295
1020-1026
2002
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1
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650379
Cater
Aspartic proteinase inhibitors ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1596
76-82
2002
-
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2
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6
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1
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1
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652130
Phylip
The potency and specificity of ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
2023-2030
2001
-
1
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1
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1
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1
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1
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1
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35
-
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1
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1
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1
35
-
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1
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1
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653778
Tello-Solis
-
Effect of the pH in the confor ...
Aspergillus phoenicis
Protein Pept. Lett.
8
101-108
2001
-
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1
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1
1
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649129
Badasso
Purification, co-crystallizati ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
56
915-917
2000
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652836
Cronin
X-ray structures of five renin ...
Saccharomyces cerevisiae
J. Mol. Biol.
303
745-760
2000
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1
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6
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1
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3
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1
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6
6
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1
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653309
Li
The aspartic proteinase from S ...
Saccharomyces cerevisiae
Nat. Struct. Biol.
7
113-117
2000
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1
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3
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3
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651749
Kondo
Substrate specificities and ki ...
Saccharomyces cerevisiae
J. Biochem.
124
141-147
1998
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1
34
1
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36
-
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36
1
1
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1
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34
1
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1
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1
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36
-
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36
1
1
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30705
Pedersen
Characterization of proteinase ...
Saccharomyces cerevisiae
Biotechnol. Appl. Biochem.
18
377-388
1993
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30707
Badasso
Crystallization and preliminar ...
Saccharomyces cerevisiae
J. Mol. Biol.
232
701-703
1993
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30708
Faust
Expression of the yeast aspart ...
Saccharomyces cerevisiae
J. Biol. Chem.
264
479-488
1989
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30710
Nowak
Purification and properties of ...
Saccharomyces cerevisiae
Can. J. Microbiol.
35
295-303
1989
1
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3
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1
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30711
Dreyer
Substrate specificity of prote ...
Saccharomyces cerevisiae
Carlsberg Res. Commun.
54
85-97
1989
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30696
Ammerer
PEP4 gene of Saccharomyces cer ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
6
2490-2499
1986
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1
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30699
Rothman
Overproduction-induced misloca ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
83
3248-3252
1986
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1
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30712
Dreyer
-
Primary structure of the aspar ...
Saccharomyces cerevisiae
Carlsberg Res. Commun.
51
27-41
1986
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30701
Dreyer
The selectivity of action of t ...
Saccharomyces cerevisiae
Biochem. J.
231
777-779
1985
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1
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30702
Dreyer
-
Partial primary structure and ...
Saccharomyces cerevisiae
Biochem. Soc. Trans.
13
1142-1143
1985
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5
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5
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30706
Yokosawa
Purification and fluorometric ...
Saccharomyces cerevisiae
Anal. Biochem.
134
210-215
1983
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1
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1
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2
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1
1
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30698
Mechler
In vivo biosynthesis of the va ...
Saccharomyces cerevisiae
J. Biol. Chem.
257
11203-11206
1982
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1
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30709
Magni
Bakers yeast protease A purifi ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
213
426-433
1982
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2
1
1
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4
1
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3
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1
1
4
1
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3
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30700
Mechler
Analysis of proteinase A funct ...
Saccharomyces cerevisiae
Eur. J. Biochem.
121
47-52
1981
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30697
Meussendoerffer
Purification and properties of ...
Saccharomyces cerevisiae
J. Biol. Chem.
255
12087-12093
1980
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1
4
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1
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3
1
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2
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3
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30703
Jusic
Inactivation of yeast enzymes ...
Saccharomyces cerevisiae
Hoppe-Seyler's Z. Physiol. Chem.
357
735-740
1976
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4
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30704
Huse
Differences in the degradation ...
Saccharomyces cerevisiae
Biochem. J.
155
721-723
1976
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1
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