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Literature summary for 3.4.23.24 extracted from

  • Dostal, J.; Brynda, J.; Hruskova-Heidingsfeldova, O.; Sieglova, I.; Pichova, I.; Rezacova, P.
    The crystal structure of the secreted aspartic protease 1 from Candida parapsilosis in complex with pepstatin A (2009), J. Struct. Biol., 167, 145-152.
    View publication on PubMed
Search for more literature for 3.4.23.24

Crystallization (Commentary)

Crystallization (Comment) Organism
Sapp1p in complex with pepstatin A, hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.0, 2.0 M ammonium sulfate and 10% (v/v) glycerol, at 19°C Candida parapsilosis

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A
-
 Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis
-
-
-
Candida parapsilosis P-69
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, MonoS column chromatography, and MonoP column chromatography Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabcyl-Glu-His-Val-Lys-Leu-Val-Glu-EDANS + H2O
-
 Candida parapsilosis dabcyl-Glu-His-Val-Lys-Leu + Val-Glu-EDANS
-
 ?
dabcyl-Glu-His-Val-Lys-Leu-Val-Glu-EDANS + H2O
-
 Candida parapsilosis P-69 dabcyl-Glu-His-Val-Lys-Leu + Val-Glu-EDANS
-
 ?

Subunits

Subunits Comment Organism
monomer the biologically relevant unit of Sapp1p is a monomer, gel filtration Candida parapsilosis
tetramer the tetrameric assembly of Sapp1p is a crystallization artifact Candida parapsilosis

Synonyms

Synonyms Comment Organism
Sapp1p
-
 Candida parapsilosis
secreted aspartic protease 1
-
 Candida parapsilosis