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Literature summary for 3.4.23.24 extracted from
- Extracellular acid and alkaline proteases from Candida olea (1987), J. Gen. Microbiol., 133, 1461-1469.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(4-nitrophenoxy)propane | not | Candida olea |  |
| Diazoacetylnorleucine methyl ester | - |
Candida olea | |
| additional information | compounds inhibiting serine, thiol or metallo proteases | Candida olea | |
| pepstatin | - |
Candida olea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29500 | - |
1 * 29500, Candida olea, SDS-PAGE | Candida olea |
| 30900 | - |
Candida olea, gel filtration | Candida olea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida olea | - |
148 | - |
| Candida olea 148 | - |
148 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 11% carbohydrate | Candida olea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida olea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Candida olea |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29500, Candida olea, SDS-PAGE | Candida olea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
denatured hemoglobin | Candida olea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
42 | 0°C: 5-10% of activity maximum, 42°C: activity maximum | Candida olea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 3.2, 60 min, slight loss of activity | Candida olea |
| 46 | - |
inactivation above | Candida olea |
| 60 | - |
almost 80% loss of activity after 10 min, complete loss of activity after 20 min | Candida olea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
denatured hemoglobin | Candida olea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
10 min, over 70% loss of activity | Candida olea |
| 9 | - |
10 min, complete loss of activity | Candida olea |
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Release 2023.1 (January 2023)
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