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Literature summary for 3.4.23.23 extracted from
- Acid protease of Mucor miehei (1970), Methods Enzymol., 19, 459-460.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Urea, pH 6.0, 11 h stable | Rhizomucor miehei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 6% covalently bound carbohydrate (hexosamine and neutral hexoses) | Rhizomucor miehei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Rhizomucor miehei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specificity against aromatic bulky or hydrophobic amino acid residues at both sides of the splitting point | Rhizomucor miehei | ? | - |
? |  |
| Oxidized insulin B-chain + H2O | hydrolysis at: Phe1-Val2, Ala14-Leu15, Leu15-Tyr16, Tyr16-Leu17, Phe24-Phe25, Phe25-Tyr26, Tyr26-Thr27 | Rhizomucor miehei | Hydrolyzed oxidized B-chain of insulin | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
hemoglobin | Rhizomucor miehei |
| 4 | - |
insulin B-chain | Rhizomucor miehei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | 38°C, 8 days, more than 90% of the activity retained | Rhizomucor miehei |
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Release 2023.1 (January 2023)
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