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Literature summary for 3.4.23.21 extracted from

  • Dunn, B.M.
    Rhizopuspepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 108-111.
No PubMed abstract available
Search for more literature for 3.4.23.21

Application

Application Comment Organism
molecular biology rhizopuspepsin is a good model enzyme to investigate acid proteinases Rhizopus microsporus var. chinensis

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination Rhizopus arrhizus
DNA sequence determination Rhizopus niveus
expresssion in Escherichia coli in inclusion bodies Rhizopus microsporus var. chinensis

Inhibitors

Inhibitors Comment Organism Structure
additional information development of petidomimetic inhibitors Rhizopus microsporus var. chinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 KPVSY(4-NO2-F)RL
-
 Rhizopus microsporus var. chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Rhizopus arrhizus
-
-
extracellular the enzyme is secreted Rhizopus microsporus var. chinensis
-
-
extracellular the enzyme is secreted Rhizopus niveus
-
-

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-
Rhizopus microsporus var. chinensis
-
-
-
Rhizopus niveus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture medium Rhizopus arrhizus
native enzyme from culture medium Rhizopus niveus
native enzyme from culture medium by ammonium sulfate precipitation and pepstatin affinity chromatography, further separation of isozymes by isoelectric focusing, recombinant enzyme from Escherichia coli inclusion bodies Rhizopus microsporus var. chinensis

Renatured (Commentary)

Renatured (Comment) Organism
solubilization and refolding of recombinant enzyme from Escherichia coli inclusion bodies Rhizopus microsporus var. chinensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhizopus microsporus var. chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. insulin B chain, cleavage site specificity Rhizopus arrhizus L-Phe + VNQH + LCGSH + L-Leu + L-Val + L-Glu + L-Ala + L-Leu + L-Tyr + LVCG + ERG + L-Phe + L-Phe + YTPKA
-
 ?
FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. insulin B chain, cleavage site specificity Rhizopus microsporus var. chinensis L-Phe + VNQH + LCGSH + L-Leu + L-Val + L-Glu + L-Ala + L-Leu + L-Tyr + LVCG + ERG + L-Phe + L-Phe + YTPKA
-
 ?
KPRRPYILKRGSYYY + H2O synthetic neurotensin-like peptide, cleavage site specificity Rhizopus microsporus var. chinensis KPRRPYIL + KRGSYYY
-
 ?
KPVSY(4-NO2-F)RL + H2O
-
 Rhizopus microsporus var. chinensis ?
-
 ?
additional information very broad substrate specificity, overview Rhizopus arrhizus ?
-
 ?
additional information very broad substrate specificity, overview Rhizopus microsporus var. chinensis ?
-
 ?
additional information very broad substrate specificity, overview Rhizopus niveus ?
-
 ?
Z-Ala-Ala-Lys-Ala-Ala-Ala + H2O
-
 Rhizopus microsporus var. chinensis Z-Ala-Ala-Lys + Ala-Ala-Ala
-
 ?

Subunits

Subunits Comment Organism
More structure analysis Rhizopus microsporus var. chinensis

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the A1 peptidase family Rhizopus arrhizus
More the enzyme belongs to the A1 peptidase family Rhizopus microsporus var. chinensis
More the enzyme belongs to the A1 peptidase family Rhizopus niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55
-
 KPVSY(4-NO2-F)RL
-
 Rhizopus microsporus var. chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.9 4.5 varying maximum dependent on the substrate Rhizopus microsporus var. chinensis

pI Value

Organism Comment pI Value Maximum pI Value
Rhizopus microsporus var. chinensis isozyme 1
-
 5.1
Rhizopus microsporus var. chinensis isozyme 2
-
 5.8