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Literature summary for 3.4.23.21 extracted from

  • Bhatt, D.; Dunn, B.M.
    Chimeric aspartic proteinases and active site binding (2000), Bioorg. Chem., 28, 374-393.
    View publication on PubMed
Search for more literature for 3.4.23.21

Application

Application Comment Organism
medicine design of a selective and potent inhibitor is crucial since aspartic proteinases known to be involved in many diseases such as cancer and malaria Rhizopus sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rhizopus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
chimeric enzymes, containing domains of porcine pepsinogen Rhizopus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ac-NA-Nva-3-hydroxy 4-amino 6-methyl heptanoic acid-Nva-NA-amide
-
 Rhizopus sp.
Ac-NA-Nva-3-hydroxy 4-amino 6-methyl heptanoyl-Nva-NA-amide
-
 Rhizopus sp.
Ac-NA-Val-3-hydroxy 4-amino 6-methyl heptanoic acid-2-aminobutanoyl-NA-amide
-
 Rhizopus sp.
Ac-NA-Val-3-hydroxy 4-amino 6-methyl heptanoyl-Abu-NA-amide
-
 Rhizopus sp.
pepstatin A
-
 Rhizopus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 KPIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.003
-
 KPIDF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.003
-
 KPISF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.004
-
 KPALF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.004
-
 KPLEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.006
-
 KPIAF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.006
-
 KPILF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.006
-
 KAIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.007
-
 KPSEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.007
-
 KLIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.008
-
 KPAEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.008
-
 KPREF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.009
-
 KSIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.01
-
 KPDEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.013
-
 KPALF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.047
-
 KPISF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.056
-
 KPILF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.057
-
 KPIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.065
-
 KPDEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.089
-
 KPAEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.101
-
 KPLEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.108
-
 KPSEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.139
-
 KLIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.157
-
 KPIAF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.21
-
 KSIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.211
-
 KPIDF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
0.269
-
 KAIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.

Organism

Organism UniProt Comment Textmining
Rhizopus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
KAIEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KAIEF + p-nitrophenylalanine-RL
-
 ?
KLIEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KLIEF + p-nitrophenylalanine-RL
-
 ?
KPAEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPAEF + p-nitrophenylalanine-RL
-
 ?
KPALF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPALF + p-nitrophenylalanine-RL
-
 ?
KPDEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPDEF + p-nitrophenylalanine-RL
-
 ?
KPIAF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPIAF + p-nitrophenylalanine-RL
-
 ?
KPIDF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPIDF + p-nitrophenylalanine-RL
-
 ?
KPIEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPIEF + p-nitrophenylalanine-RL
-
 ?
KPILF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPILF + p-nitrophenylalanine-RL
-
 ?
KPISF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPISF + p-nitrophenylalanine-RL
-
 ?
KPLEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPLEF + p-nitrophenylalanine-RL
-
 ?
KPREF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPREF + p-nitrophenylalanine-RL
-
 ?
KPSEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KPSEF + p-nitrophenylalanine-RL
-
 ?
KSIEF p-nitrophenylalanine-RL + H2O
-
 Rhizopus sp. KSIEF + p-nitrophenylalanine-RL
-
 ?
Lys-Pro-Ala-Lys-Phe-(NO2)Phe-Arg-Leu + H2O
-
 Rhizopus sp. Lys-Pro-Ala-Lys-Phe + (NO2)Phe-Arg-Leu
-
 ?
Lys-Pro-Ile-Glu-Phe-(NO2)Phe-Arg-Leu + H2O
-
 Rhizopus sp. Lys-Pro-Ile-Glu-Phe + (NO2)Phe-Arg-Leu
-
 ?

Synonyms

Synonyms Comment Organism
rhizopuspepsinogen
-
 Rhizopus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 KPILF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
0.77
-
 KPIDF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
1.8
-
 KSIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
3
-
 KPISF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
3
-
 KPIAF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
3
-
 KPILF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
3
-
 KPDEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
4
-
 KPISF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
5
-
 KAIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
5
-
 KLIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
5
-
 KPIDF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
6
-
 KPIEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
6
-
 KPSEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
6.08
-
 KPIDF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
7
-
 KPSEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
7
-
 KPLEF p-nitrophenylalanine-RL pH 3.0, 37°C, wild-type and chimeric enzyme Rhizopus sp.
8
-
 KPDEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
9
-
 KPALF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
13
-
 KPALF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
13
-
 KSIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
17
-
 KPIAF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
18
-
 KPREF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
21
-
 KPAEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
22
-
 KPAEF p-nitrophenylalanine-RL pH 3.0, 37°C Rhizopus sp.
38
-
 KLIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
41
-
 KPIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.
46
-
 KAIEF p-nitrophenylalanine-RL pH 3.0, 37°C, chimeric enzyme Rhizopus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Rhizopus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000006
-
 pepstatin A pH 3.0, 37°C Rhizopus sp.
0.0000029
-
 Ac-NA-Val-3-hydroxy 4-amino 6-methyl heptanoic acid-2-aminobutanoyl-NA-amide pH 3.0, 37°C Rhizopus sp.
0.000077
-
 Ac-NA-Nva-3-hydroxy 4-amino 6-methyl heptanoic acid-Nva-NA-amide pH 3.0, 37°C Rhizopus sp.