BRENDA - Enzyme Database show
show all sequences of 3.4.23.20

Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S3 to k(cat)

Cao, Q.N.; Stubbs, M.; Ngo, K.Q.; Ward, M.; Cunningham, A.; Pai, E.F.; Tu, G.C.; Hofmann, T.; Protein Sci. 9, 991-1001 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene pepA, nucleotide sequence determined, inserted into expression vector pGPT-pyrG1, expressed in an aspartic proteinase-free strain of Aspergillus niger var. awamori
Penicillium janthinellum
Engineering
Amino acid exchange
Commentary
Organism
T219A
constructed by cassette insertion
Penicillium janthinellum
T219G
constructed by cassette insertion
Penicillium janthinellum
T219S
constructed by cassette insertion
Penicillium janthinellum
T219V
constructed by cassette insertion
Penicillium janthinellum
Inhibitors
Inhibitors
Commentary
Organism
Structure
pepstatin
-
Penicillium janthinellum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.32
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.37
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, and mutant T219S
Penicillium janthinellum
0.39
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291S
Penicillium janthinellum
0.4
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.4
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.41
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.42
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.46
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.46
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.46
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.48
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.5
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.5
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.51
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.54
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.55
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291G
Penicillium janthinellum
0.55
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.57
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291V
Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.59
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.6
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.6
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G and T219S
Penicillium janthinellum
0.65
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.68
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291A
Penicillium janthinellum
0.71
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V and T219A
Penicillium janthinellum
0.74
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.75
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.77
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.8
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.83
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.83
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33700
34000
mass spectrometry
Penicillium janthinellum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Penicillium janthinellum
-
-
-
Penicillium janthinellum NRRL 905
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
PencillopepsinJT2, glycosylated form of the active enzyme
Penicillium janthinellum
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Penicillium janthinellum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0016
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.0019
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, and mutant T219V
Penicillium janthinellum
0.002
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2 and mutant T219A
Penicillium janthinellum
0.003
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.003
-
Ac-Ala-Lys-Ala-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.003
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G; pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.003
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.004
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.005
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.005
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.0054
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.042
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.046
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.05
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.07
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.09
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.09
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.11
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.12
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.12
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.13
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.14
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.15
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.17
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.23
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.24
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.29
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.3
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A; pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.33
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291V
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291A
Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.43
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V and T219G
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
3.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
14.9
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
15
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
16.1
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291S
Penicillium janthinellum
34
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
40
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00000001
-
pepstatin
pH 5.0, 37°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.0000004
-
pepstatin
pH 5.0, 37°C, mutant T219S
Penicillium janthinellum
0.00055
-
pepstatin
pH 5.0, 37°C, mutant T219A
Penicillium janthinellum
0.0016
-
pepstatin
pH 5.0, 37°C, mutant T219V
Penicillium janthinellum
0.0018
-
pepstatin
pH 5.0, 37°C, mutant T219G
Penicillium janthinellum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pepA, nucleotide sequence determined, inserted into expression vector pGPT-pyrG1, expressed in an aspartic proteinase-free strain of Aspergillus niger var. awamori
Penicillium janthinellum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
T219A
constructed by cassette insertion
Penicillium janthinellum
T219G
constructed by cassette insertion
Penicillium janthinellum
T219S
constructed by cassette insertion
Penicillium janthinellum
T219V
constructed by cassette insertion
Penicillium janthinellum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
pepstatin
-
Penicillium janthinellum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00000001
-
pepstatin
pH 5.0, 37°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.0000004
-
pepstatin
pH 5.0, 37°C, mutant T219S
Penicillium janthinellum
0.00055
-
pepstatin
pH 5.0, 37°C, mutant T219A
Penicillium janthinellum
0.0016
-
pepstatin
pH 5.0, 37°C, mutant T219V
Penicillium janthinellum
0.0018
-
pepstatin
pH 5.0, 37°C, mutant T219G
Penicillium janthinellum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.32
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.37
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, and mutant T219S
Penicillium janthinellum
0.39
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291S
Penicillium janthinellum
0.4
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.4
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.41
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.42
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.46
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.46
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.46
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.48
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.48
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.5
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.5
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.51
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.54
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.55
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291G
Penicillium janthinellum
0.55
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.57
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291V
Penicillium janthinellum
0.59
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.59
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type Pencillopepsin JT-2
Penicillium janthinellum
0.6
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.6
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G and T219S
Penicillium janthinellum
0.65
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.68
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291A
Penicillium janthinellum
0.71
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V and T219A
Penicillium janthinellum
0.74
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.75
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.77
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.8
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.83
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.83
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33700
34000
mass spectrometry
Penicillium janthinellum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
PencillopepsinJT2, glycosylated form of the active enzyme
Penicillium janthinellum
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Penicillium janthinellum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Ala-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe- Ala-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Ac-Lys-(NO2)Phe-Ala-Ala-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum
?
-
653829
Penicillium janthinellum
?
Ac-Lys-(NO2)Phe-amide + H2O
-
653829
Penicillium janthinellum NRRL 905
?
-
653829
Penicillium janthinellum NRRL 905
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0016
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.0019
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, and mutant T219V
Penicillium janthinellum
0.002
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2 and mutant T219A
Penicillium janthinellum
0.003
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.003
-
Ac-Ala-Lys-Ala-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.003
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G; pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.003
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.004
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.005
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.005
-
Ac-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.0054
-
Ac-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.042
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.046
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.05
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.07
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.09
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.09
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.11
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.12
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.12
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.13
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.14
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.15
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.17
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.23
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.24
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.29
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.3
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V
Penicillium janthinellum
0.3
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A; pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.33
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291V
Penicillium janthinellum
0.35
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291A
Penicillium janthinellum
0.36
-
Ac-Ala-Ala-Lys-(NO2)Phe-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.4
-
Ac-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
0.43
-
Ac-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291G
Penicillium janthinellum
0.45
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219A
Penicillium janthinellum
0.45
-
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219V and T219G
Penicillium janthinellum
0.5
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219G
Penicillium janthinellum
0.6
-
Ac-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
3.8
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
14.9
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T219S
Penicillium janthinellum
15
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
16.1
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, mutant T291S
Penicillium janthinellum
34
-
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
40
-
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
pH 5.0, 25°C, wild-type pencillopepsin JT-2
Penicillium janthinellum
Other publictions for EC 3.4.23.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670005
Vidossich
Binding of phosphinate and pho ...
Penicillium janthinellum
J. Phys. Chem. B
110
1437-1442
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668813
Hofmann
-
Penicillopepsin ...
Penicillium camemberti, Penicillium duponti, Penicillium duponti K1014, Penicillium janthinellum, Penicillium roqueforti
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
99-104
2004
-
-
1
1
4
-
4
1
-
-
5
-
-
7
-
3
4
-
-
-
-
-
16
5
-
-
-
1
-
-
-
-
-
2
-
-
-
3
-
1
4
-
-
4
-
1
-
-
5
-
-
-
5
5
-
-
-
-
16
6
-
-
-
1
-
-
-
2
-
-
-
-
-
-
653829
Cao
Penicillopepsin-JT2, a recombi ...
Penicillium janthinellum, Penicillium janthinellum NRRL 905
Protein Sci.
9
991-1001
2000
-
-
1
-
4
-
1
47
-
-
1
-
-
5
-
1
1
-
-
-
-
-
15
-
-
-
-
47
-
-
-
-
5
-
-
-
-
1
-
-
4
-
-
1
5
47
-
-
1
-
-
-
1
1
-
-
-
-
15
-
-
-
-
47
-
-
-
-
-
-
-
-
-
-
649272
Fraser
Overcoming the unfavourable en ...
Penicillium sp.
Adv. Exp. Med. Biol.
436
355-359
1998
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649841
Khan
Lowering the entropic barrier ...
Penicillium janthinellum
Biochemistry
37
16839-16845
1998
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30601
James
Crystallographic analysis of t ...
Penicillium janthinellum
Biochemistry
31
3872-3886
1992
-
-
-
1
-
-
1
-
-
-
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30602
Fraser
Crystallographic analysis of t ...
Penicillium janthinellum
Biochemistry
31
5201-5214
1992
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30588
Hofmann
Effect of secondary substrate ...
Penicillium janthinellum
Biochemistry
27
1140-1146
1988
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30589
Dunn
A systematic series of synthet ...
Penicillium janthinellum
Biochem. J.
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1986
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30597
Blum
Penicillopepsin, the aspartic ...
Penicillium janthinellum
Biochem. Soc. Trans.
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1985
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30598
James
Stereochemical analysis of pep ...
Penicillium janthinellum
Biochemistry
24
3701-3713
1985
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30599
Hofmann
Effect of pH on the activities ...
Penicillium janthinellum
Biochemistry
23
635-643
1984
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30600
James
Structure and refinement of pe ...
Penicillium janthinellum
J. Mol. Biol.
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1983
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30603
Houmard
Further characterization of th ...
Penicillium roqueforti
Biochimie
61
979-982
1979
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30587
Hsu
Penicillopepsin from Penicilli ...
Penicillium janthinellum
Nature
266
140-145
1977
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30590
James
Mechanism of acid protease cat ...
Penicillium janthinellum
Nature
267
808-813
1977
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30592
Wang
Acyl and amino intermediates i ...
Penicillium janthinellum
Can. J. Biochem.
55
286-294
1977
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30585
Hofmann
Penicillopepsin ...
Penicillium janthinellum
Methods Enzymol.
45
434-452
1976
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9
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30586
Emi
Purification and properties of ...
Penicillium duponti, Penicillium duponti K 1014
Biochemistry
15
842-848
1976
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30593
Hsu
The crystal structure of penic ...
Penicillium janthinellum
Biochem. Biophys. Res. Commun.
72
363-368
1976
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30594
Mabrouk
-
A rennin-like enzyme from Peni ...
Penicillium expansum
Agric. Biol. Chem.
40
419-420
1976
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30604
Gripon
Inactivation of Penicillium ro ...
Penicillium roqueforti
Biochimie
58
747-749
1976
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4
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30591
Mains
The inactivation of penicillop ...
Penicillium janthinellum
Can. J. Biochem.
52
1018-1023
1974
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30595
Hashimoto
Some properties of acid protea ...
Penicillium duponti, Penicillium duponti K 1014
Appl. Microbiol.
25
578-583
1973
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30596
Hashimoto
Production and purification of ...
Penicillium duponti, Penicillium duponti K 1014
Appl. Microbiol.
25
584-588
1973
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