Inhibitors | Comment | Organism | Structure |
---|---|---|---|
synthetic propeptides | wild-type sequence Ala1-Asn41 and diverse truncated or mutated propeptides, overview, Ala1-Asn41 shows specific competitive inhibition, while mutants, especially R19A and H20A, show weak inhibitory effects, detailed overview | Aspergillus niger var. macrosporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, thermodynamics, overview | Aspergillus niger var. macrosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger var. macrosporus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is synthesized as preproform and performs autoprocessing to the mature enzyme removing the 41-amino acid propeptide and an 11-amino acid intervening peptide | Aspergillus niger var. macrosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | - |
Aspergillus niger var. macrosporus | DRVYIH + Pro-Phe | - |
? | |
substance P + H2O | - |
Aspergillus niger var. macrosporus | RPKPQQFF + Gly-Leu-Met | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGP | - |
Aspergillus niger var. macrosporus |
aspergilloglutamic peptidase | - |
Aspergillus niger var. macrosporus |
More | the enzyme belongs to the G1 peptidase family | Aspergillus niger var. macrosporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus niger var. macrosporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability overview, kinetics, thermodynamics, thermal unfolding is reversible at pH 5.0-6.0, overview, the wild-type propeptide stabilizes the enzyme, effects of truncated or mutated propeptides, overview | Aspergillus niger var. macrosporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Aspergillus niger var. macrosporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Aspergillus niger var. macrosporus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics of truncated or mutated propeptides, detailed overview | Aspergillus niger var. macrosporus | |
0.000027 | - |
synthetic propeptide Ala1-Asn24 | pH 4.0, 30°C | Aspergillus niger var. macrosporus |