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Literature summary for 3.4.23.19 extracted from

  • Huang, X.P.; Kagami, N.; Inoue, H.; Kojima, M.; Kimura, T.; Makabe, O.; Suzuki, K.; Takahashi, K.
    Identification of a glutamic acid and an aspartic acid residue essential for catalytic activity of aspergillopepsin II, a non-pepsin type acid proteinase (2000), J. Biol. Chem., 275, 26607-26614.
    View publication on PubMed
Search for more literature for 3.4.23.19

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant pro-enzymes expressed in Escherichia coli Aspergillus niger

Protein Variants

Protein Variants Comment Organism
D123A site-directed mutagenesis, activity is almost at background levels Aspergillus niger
D123E site-directed mutagenesis, activity not detectable, below 0.2% Aspergillus niger
D123E/E219D site-directed mutagenesis, weak activity against acid-denatured hemoglobin Aspergillus niger
D123N site-directed mutagenesis Aspergillus niger
D125N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D137N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D148N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D160N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D170N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D208N site-directed mutagenesis Aspergillus niger
D220N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
D225N site-directed mutagenesis Aspergillus niger
D254N site-directed mutagenesis Aspergillus niger
E128Q site-directed mutagenesis Aspergillus niger
E141Q site-directed mutagenesis Aspergillus niger
E152Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
E177Q site-directed mutagenesis Aspergillus niger
E189Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
E206Q site-directed mutagenesis Aspergillus niger
E211Q site-directed mutagenesis Aspergillus niger
E2154A site-directed mutagenesis Aspergillus niger
E219A site-directed mutagenesis, activity is almost at background levels Aspergillus niger
E219D site-directed mutagenesis, activity not detectable, below 0.2% Aspergillus niger
E219Q site-directed mutagenesis Aspergillus niger
E222Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 var.macrosporus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Aspergillus niger ?
-
 ?
Milk casein + H2O
-
 Aspergillus niger ?
-
 ?

Synonyms

Synonyms Comment Organism
acid proteinase A
-
 Aspergillus niger
non-pepsin type acid proteinase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 65 large decrease in activity at 60-65°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6
-
 milk casein as substrate Aspergillus niger
5.3
-
 hemoglobin as substrate Aspergillus niger