Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.18 extracted from

  • Takenaka, S.; Umeda, M.; Senba, H.; Koyama, D.; Tanaka, K.; Yoshida, K.; Doi, M.
    Heterologous expression and characterisation of the Aspergillus aspartic protease involved in the hydrolysis and decolorisation of red-pigmented proteins (2017), J. Sci. Food Agric., 97, 95-101 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Aspergillus pseudoglaucus

General Stability

General Stability Organism
the enzyme is stable in 50 mM KCl-HCl buffer (pH 1.2 and 2.0), sodium potassium tartrate buffer (pH 3.0), 50 mM sodium acetate buffer (pH 4.0-5.0), and 50 mM sodium/potassium phosphate buffer (pH 6.0-7.0) Aspergillus pseudoglaucus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by H2O2 Aspergillus pseudoglaucus
polyoxyethylene lauryl ether 88.7% residual activity after 3 h at 1% (v/v) Aspergillus pseudoglaucus
sodium laurylbenzenesulfonic acid 5.5% residual activity after 3 h at 1% (v/v) Aspergillus pseudoglaucus
sodium perborate 92.1% residual activity after 3 h at 1% (v/v) Aspergillus pseudoglaucus

Organism

Organism UniProt Comment Textmining
Aspergillus pseudoglaucus
-
-
-
Aspergillus pseudoglaucus MK82
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation Aspergillus pseudoglaucus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth Aspergillus pseudoglaucus ?
-
?
additional information the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth Aspergillus pseudoglaucus MK82 ?
-
?
Myoglobin + H2O
-
Aspergillus pseudoglaucus ?
-
?
Myoglobin + H2O
-
Aspergillus pseudoglaucus MK82 ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, mature enzyme, SDS-PAGE Aspergillus pseudoglaucus
? x * 47000, proenzyme, SDS-PAGE Aspergillus pseudoglaucus

Synonyms

Synonyms Comment Organism
aspartic protease
-
Aspergillus pseudoglaucus
PepA
-
Aspergillus pseudoglaucus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus pseudoglaucus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme maintains 80% activity when incubated at 50°C and pH 3.0 for 20 min Aspergillus pseudoglaucus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
Aspergillus pseudoglaucus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the protease is relatively stable at pH 5.0, except in buffer containing sodium laurylbenzenesulfonic acid. However, at pH 8.0, whether in the absence or presence of reagents for 1 h, it loses almost all activity Aspergillus pseudoglaucus