Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Aspergillus pseudoglaucus |
General Stability | Organism |
---|---|
the enzyme is stable in 50 mM KCl-HCl buffer (pH 1.2 and 2.0), sodium potassium tartrate buffer (pH 3.0), 50 mM sodium acetate buffer (pH 4.0-5.0), and 50 mM sodium/potassium phosphate buffer (pH 6.0-7.0) | Aspergillus pseudoglaucus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by H2O2 | Aspergillus pseudoglaucus | |
polyoxyethylene lauryl ether | 88.7% residual activity after 3 h at 1% (v/v) | Aspergillus pseudoglaucus | |
sodium laurylbenzenesulfonic acid | 5.5% residual activity after 3 h at 1% (v/v) | Aspergillus pseudoglaucus | |
sodium perborate | 92.1% residual activity after 3 h at 1% (v/v) | Aspergillus pseudoglaucus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus pseudoglaucus | - |
- |
- |
Aspergillus pseudoglaucus MK82 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation | Aspergillus pseudoglaucus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth | Aspergillus pseudoglaucus | ? | - |
? | |
additional information | the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth | Aspergillus pseudoglaucus MK82 | ? | - |
? | |
Myoglobin + H2O | - |
Aspergillus pseudoglaucus | ? | - |
? | |
Myoglobin + H2O | - |
Aspergillus pseudoglaucus MK82 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, mature enzyme, SDS-PAGE | Aspergillus pseudoglaucus |
? | x * 47000, proenzyme, SDS-PAGE | Aspergillus pseudoglaucus |
Synonyms | Comment | Organism |
---|---|---|
aspartic protease | - |
Aspergillus pseudoglaucus |
PepA | - |
Aspergillus pseudoglaucus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus pseudoglaucus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme maintains 80% activity when incubated at 50°C and pH 3.0 for 20 min | Aspergillus pseudoglaucus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Aspergillus pseudoglaucus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the protease is relatively stable at pH 5.0, except in buffer containing sodium laurylbenzenesulfonic acid. However, at pH 8.0, whether in the absence or presence of reagents for 1 h, it loses almost all activity | Aspergillus pseudoglaucus |