Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.18 extracted from

  • Niyonzima, F.N.; More, S.S.
    Purification and characterization of detergent-compatible protease from Aspergillus terreus gr (2015), 3 Biotech, 5, 61-70 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.18

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity remains unaltered in the presence of H2O2 and NaClO Aspergillus terreus
SDS
-
 Aspergillus terreus
Triton-X100
-
 Aspergillus terreus
Tween-80
-
 Aspergillus terreus

General Stability

General Stability Organism
the protease is 100 % stable and compatible for 2 h at 60°C with all the detergents tested except for Super wheel, and a retention of 83.98, 85.50, 85.57, 87.14 and 89.16 % is recorded after 24 h for Super wheel, More choice, Ariel, Henko and Surf excel, respectively Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
DAN 91.7% residual activity at 5 mM Aspergillus terreus
dithiothreitol 90.1% residual activity at 5 mM Aspergillus terreus
EDTA 87.3% residual activity at 5 mM Aspergillus terreus
Fe3+ 81.1% residual activity at 10 mM Aspergillus terreus
Hg2+ 74.8% residual activity at 10 mM Aspergillus terreus
IAA 94.8% residual activity at 5 mM Aspergillus terreus
K+ 97.4% residual activity at 10 mM Aspergillus terreus
Mn2+ 85.9% residual activity at 10 mM Aspergillus terreus
additional information not inhibited by Fe2+ and Cu2+ Aspergillus terreus
N-ethylmaleimide 88.8% residual activity at 5 mM Aspergillus terreus
Na+ 92.54% residual activity at 10 mM Aspergillus terreus
NaN3 78% residual activity at 5 mM Aspergillus terreus
NBS 49.7% residual activity at 5 mM Aspergillus terreus
phenylmethylsulfonyl fluoride 16.3% residual activity at 5 mM Aspergillus terreus
TLCK 93.3% residual activity at 5 mM Aspergillus terreus
Urea 95.5% residual activity at 5 mM Aspergillus terreus
Zn2+ 89.1% residual activity at 10 mM Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 182.6% activity at 10 mM Aspergillus terreus
Co2+ 121.1% activity at 10 mM Aspergillus terreus
Mg2+ 152.2% activity at 10 mM Aspergillus terreus
Na+ 102.88% activity at 10 mM Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus gr.
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is 100 % stable in the presence of bleaching and oxidizing agents Aspergillus terreus

Purification (Commentary)

Purification (Comment) Organism
concanavalin A-agarose column chromatography Aspergillus terreus

Storage Stability

Storage Stability Organism
4, 7 and 9% of the enzyme's initial activity are lost after 40 days at 4, 28 and -20°C, respectively Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O highest activity Aspergillus terreus ?
-
 ?
casein + H2O highest activity Aspergillus terreus gr. ?
-
 ?
Gelatin + H2O
-
 Aspergillus terreus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 16000, SDS-PAGE Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Aspergillus terreus

pH Range

pH Minimum pH Maximum Comment Organism
8 12 the enzyme is active over a broad alkaline pH range of 8.0-12.0 Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 12 the protease demonstrates excellent stability at pH range 8.0-12.0 for 30 min. The enzyme remains unaltered at pH 11.0 and 12.0 for 24 h at 28°C and a slight decrease in relative activity is seen at pH 10.0 after 24 h Aspergillus terreus