BRENDA - Enzyme Database show
show all sequences of 3.4.23.18

The structure and function of acid proteases. V. Comparative studies on the specific inhibition of acid proteases by diazoacetyl-DL-norleucine methyl ester, 1,2-epoxy-3-(p-nitrophenoxy) propane and pepstatin

Takahashi, K.; Chang, W.J.; J. Biochem. 80, 497-506 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Diazoacetyl-DL-norleucine methyl ester
in the presence of Cu2+; pH-profile
Aspergillus niger
Diazoacetyl-DL-norleucine methyl ester
in the presence of Cu2+; pH-profile
Aspergillus phoenicis
pepstatin
kinetics
Aspergillus niger
pepstatin
kinetics
Aspergillus phoenicis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
var. macrosporus
-
Aspergillus phoenicis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hemoglobin + H2O
-
30568
Aspergillus niger
?
-
-
-
-
Hemoglobin + H2O
-
30568
Aspergillus phoenicis
?
-
-
-
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Diazoacetyl-DL-norleucine methyl ester
in the presence of Cu2+; pH-profile
Aspergillus niger
Diazoacetyl-DL-norleucine methyl ester
in the presence of Cu2+; pH-profile
Aspergillus phoenicis
pepstatin
kinetics
Aspergillus niger
pepstatin
kinetics
Aspergillus phoenicis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hemoglobin + H2O
-
30568
Aspergillus niger
?
-
-
-
-
Hemoglobin + H2O
-
30568
Aspergillus phoenicis
?
-
-
-
-
Other publictions for EC 3.4.23.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
680281
Landbo
Protease-assisted clarificatio ...
Aspergillus niger
J. Agric. Food Chem.
54
6554-6563
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668006
Zhu
Analysis of secreted proteins ...
Aspergillus oryzae, Aspergillus oryzae RIB 40
Biosci. Biotechnol. Biochem.
68
2607-2612
2004
-
-
-
-
-
-
1
-
1
-
2
-
-
4
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
1
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668812
Ichishima
-
Aspergillopepsin I ...
Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus kawachii, Aspergillus niger, Aspergillus oryzae, Aspergillus phoenicis, Aspergillus phoenicis R-3813, Aspergillus sojae
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
92-99
2004
-
5
5
1
1
-
1
-
8
-
2
9
-
10
-
1
1
1
1
9
-
-
26
6
-
-
-
-
4
1
1
-
-
-
-
-
5
5
-
1
1
-
-
1
-
-
8
-
2
9
-
-
1
1
1
9
-
-
26
6
-
-
-
-
4
1
1
-
-
-
-
-
-
-
652904
Kamitori
Crystal structures of Aspergil ...
Aspergillus oryzae
J. Mol. Biol.
326
1503-1511
2003
-
-
-
1
-
-
1
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649170
Cho
Structure of aspergillopepsin ...
Aspergillus phoenicis
Acta Crystallogr. Sect. D
57
948-956
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30556
Lu
Molecular cloning of a cDNA fo ...
Aspergillus awamori, Aspergillus niger, Aspergillus phoenicis
Biosci. Biotechnol. Biochem.
59
954-955
1995
-
-
-
-
-
-
3
-
6
-
3
-
-
5
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
6
-
3
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30558
Tello-Solis
Effect of irreversibility on t ...
Aspergillus phoenicis
Biochem. J.
311
969-974
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
30557
Takeuchi
-
Fluorogenic substrate of Asper ...
Aspergillus phoenicis
Agric. Biol. Chem.
52
1279-1280
1988
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
30559
Majima
-
Comparative study on the speci ...
Aspergillus oryzae, Aspergillus phoenicis, Aspergillus sojae
Agric. Biol. Chem.
52
787-793
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
30560
Yagi
Purification and characterizat ...
Aspergillus kawachii
Agric. Biol. Chem.
50
1029-1033
1986
-
-
-
-
-
-
3
-
2
-
2
-
-
1
-
-
1
-
-
2
1
-
1
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
2
-
-
-
-
1
-
2
1
-
1
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
30574
Krishnan
Purification of an acid protea ...
Aspergillus niger
J. Chromatogr.
329
165-170
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30561
Bhumibhamon
-
Precipitation and characterist ...
Aspergillus phoenicis
Thai J. Agric. Sci.
15
157-172
1982
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
30562
Panneerselvam
Physico-chemical properties of ...
Aspergillus fumigatus
Ital. J. Biochem.
30
63-74
1981
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
30563
Bhumibhamon
-
Some characreristics of the ac ...
Aspergillus awamori
Thai J. Agric. Sci.
12
27-33
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
30570
Tsujita
Presence and partial character ...
Aspergillus oryzae, Aspergillus oryzae 365-U-64-1
Appl. Environ. Microbiol.
36
237-242
1978
1
-
-
-
-
-
2
-
3
-
2
-
-
3
-
-
1
-
-
2
1
-
8
-
-
-
1
-
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
2
-
-
-
-
1
-
2
1
-
8
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
30575
Tsujita
Purification and characterizat ...
Aspergillus oryzae
Eur. J. Biochem.
84
347-353
1978
6
-
-
-
-
-
3
-
1
-
3
-
-
3
-
1
1
-
-
1
2
-
3
-
-
-
1
-
2
-
1
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
1
-
3
-
-
-
1
1
-
1
2
-
3
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
30571
Tanaka
Purification of an acid protei ...
Aspergillus phoenicis
Biochim. Biophys. Acta
485
406-416
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30568
Takahashi
The structure and function of ...
Aspergillus niger, Aspergillus phoenicis
J. Biochem.
80
497-506
1976
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30569
Tsujita
Purification and characterizat ...
Aspergillus oryzae
Biochim. Biophys. Acta
445
194-204
1976
-
-
-
1
-
-
3
-
-
1
3
-
-
3
-
1
1
-
-
1
-
1
5
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
1
3
-
-
-
1
1
-
1
-
1
5
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
30572
Chang
The structure and function of ...
Aspergillus niger
J. Biochem.
80
975-981
1976
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30573
Iio
Specificity of acid proteinase ...
Aspergillus niger
Biochim. Biophys. Acta
429
912-924
1976
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
30567
Davidson
Aspergillus oryzae acid protei ...
Aspergillus oryzae
Biochem. J.
147
45-53
1975
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
1
1
-
-
-
-
-
6
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
1
-
-
-
-
6
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
30566
Morihara
Comparative specificity of mic ...
Aspergillus niger, Aspergillus niger B, Aspergillus phoenicis
Arch. Biochem. Biophys.
157
561-572
1973
-
-
-
-
-
-
-
7
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
-
2
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
22
-
2
-
-
8
1
-
-
-
-
-
-
-
-
-
30565
Kovaleva
The site of diazoacetyl inhibi ...
Aspergillus awamori
Biochem. Biophys. Res. Commun.
49
1075-1081
1972
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30564
Ichishima
-
Purification and mode of assay ...
Aspergillus phoenicis, Aspergillus phoenicis R-3813
Methods Enzymol.
19
397-406
1970
1
-
-
-
-
2
3
1
2
1
3
-
-
3
-
-
1
-
-
3
-
1
6
-
-
-
3
-
2
-
2
-
-
-
-
1
-
-
-
-
-
2
-
3
-
1
2
1
3
-
-
-
-
1
-
3
-
1
6
-
-
-
3
-
2
-
2
-
-
-
-
-
-
-