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Literature summary for 3.4.23.18 extracted from
- Aspergillus oryzae acid proteinase. Purification and properties, and formation of pi-chymotrypsin (1975), Biochem. J., 147, 45-53.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Trypsinogen | 35°C, pH 3.5 | Aspergillus oryzae |  |
| 0.18 | - |
chymotrypsinogen | 35°C, pH 3.5 | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Aspergillus oryzae |
| 39400 | - |
Aspergillus oryzae, sedimentation equilibrium meniscus depletion method | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 1-2% | Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from crude powder preparation | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Ala-Ala-Phe-Phe 3-(4-pyridyl)propyl ester + H2O | - |
Aspergillus oryzae | Benzyloxycarbonyl-Ala-Ala-Phe + Phe 3-(4-pyridyl)propyl ester | - |
? | |
| Benzyloxycarbonyl-His-Phe-Phe ethyl ester + H2O | - |
Aspergillus oryzae | Benzyloxycarbonyl-His-Phe + Phe ethyl ester | - |
? | |
| Chymotrypsinogen + H2O | cleavage site: Arg15-Ile16 | Aspergillus oryzae | Pi-Chymotrypsin | - |
? | |
| Chymotrypsinogen + H2O | bovine chymotrypsinogen | Aspergillus oryzae | Pi-Chymotrypsin | - |
? | |
| Trypsinogen + H2O | better substrate than chymotrypsinogen | Aspergillus oryzae | ? | - |
? | |
| Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
chymotrypsinogen | 35°C, pH 3.5 | Aspergillus oryzae | |
| 11.3 | - |
Trypsinogen | 35°C, pH 3.5 | Aspergillus oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 3.6 | chymotrypsin, 25°C | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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