Crystallization (Comment) | Organism |
---|---|
vapor diffusion hanging drop method, mutant enzyme complexed to darunavir or acetyl-Thr-Ile-Nle-r-Nle-Gln-Arg-NH2 | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
D25N | mutation increases the equilibrium dimer dissociation constant by a factor of more than 100fold relative to wild-type. In the absence of inhibitor, NMR studies reveal clear structural differences between wild-type and mutant enzyme in the relatively mobile P1 loop (residues 79-83) and flap regions, and differential scanning calorimetric analyses show that the mutation lowers the stabilities of both the monomer and dimer folds by 5 and 7.3°C, respectively. Complexation with acetyl-Thr-Ile-Nle-r-Nle-Gln-Arg-NH2 stabilizes both dimers, the effect on their Tm is smaller for the mutant enzyme (6.2°C) than for wild-type enzyme (8.7°C). The Tm of mutant enzyme/darunavir increases by only 3°C relative to free mutant enzyme, as compared with a 22°C increase for wild-type enzyme/darunavir. Mutation increases the ligand dissociation constant of mutant enzyme/darunavir by a factor of about 1000000 relative to wild-type enzyme/darunavir | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
darunavir | - |
Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 protease | - |
Human immunodeficiency virus 1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
complexation with acetyl-Thr-Ile-Nle-r-Nle-Gln-Arg-NH2 stabilizes dimers of wild-type enzyme and mutant enzyme D25N. The effect on their Tm is smaller for the mutant enzyme (6.2°C) than for wild-type enzyme (8.7°C). The Tm of mutant enzyme-darunavir complex increases by 3°C relative to free mutant enzyme, as compared with a 22°C increase for wild-type enzyme-darunavir complex | Human immunodeficiency virus 1 |