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Literature summary for 3.4.23.16 extracted from

  • Ridky, T.W.; Kikonyogo, A.; Leis, J.; Gulnik, S.; Copeland, T.; Erickson, J.; Wlodawer, A.; Kurinov, I.; Harrison, R.W.; Weber, I.T.
    Drug-resistant HIV-1 proteases identify enzyme residues important for substrate selection and catalytic rate (1998), Biochemistry, 37, 13835-13845.
    View publication on PubMed
Search for more literature for 3.4.23.16

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
G48V/L90M mutant exhibits wild-type preference for large hydrophobic residues, especially Phe, in the P1' substrate position Human immunodeficiency virus 1
I84V mutant exhibits wild-type preference for large hydrophobic residues, especially Phe, in the P1' substrate position Human immunodeficiency virus 1
R10K altered substrate specificity and catalytic rate compared to wild-type enzyme Human immunodeficiency virus 1
R8K significant difference in subsite selection compared to wild-type enzyme Human immunodeficiency virus 1
V32I significant difference in subsite selection compared to wild-type enzyme Human immunodeficiency virus 1
V32T mutant exhibits wild-type preference for large hydrophobic residues, especially Phe, in the P1' substrate position Human immunodeficiency virus 1
V82T/I84V mutant exhibits wild-type preference for large hydrophobic residues, especially Phe, in the P1' substrate position Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 PAFVLAEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.009
-
 PAFVLAEAMR wild-type enzyme Human immunodeficiency virus 1
0.009
-
 PAFVLAEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.01
-
 PARVLVEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.01
-
 PAFVLAEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.013
-
 PARVLFEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.013
-
 PAFVLAEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.014
-
 PARVLVEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.015
-
 PARVLVEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.016
-
 PARVLVEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.02
-
 PARVLFEAMR wild-type enzyme Human immunodeficiency virus 1
0.023
-
 PARVLIEAMR wild-type enzyme Human immunodeficiency virus 1
0.024
-
 PARVLMEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.024
-
 PARVLMEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.024
-
 PARVLVEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.025
-
 PARVLFEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.027
-
 PARVLFEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.027
-
 PARVLAEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.027
-
 PARVLIEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.03
-
 PARVLLEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.031
-
 PARVLLEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.032
-
 PARVLMEAMR wild-type enzyme Human immunodeficiency virus 1
0.033
-
 PARVLAEAMR wild-type enzyme Human immunodeficiency virus 1
0.033
-
 PARVLLEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.033
-
 PARVLFEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.033
-
 PARVLMEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.034
-
 PARVLLEAMR wild-type enzyme Human immunodeficiency virus 1
0.036
-
 PARVLMEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.044
-
 PARVLVEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.046
-
 PARVLFEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.046
-
 PARVLAEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.051
-
 PARVLAEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.054
-
 PARVLIEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.056
-
 PARVLAEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.057
-
 PARVLFEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.061
-
 PARVLLEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.061
-
 PARVLLAEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.07
-
 PARVLLEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.071
-
 PARVLIEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.072
-
 PARVLAEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.09
-
 PARVLIEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.1
-
 PARVLVEAMR wild-type enzyme Human immunodeficiency virus 1
0.105
-
 PARVLAEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.108
-
 PARVLIEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.129
-
 PARVLIEAMR mutant enzyme V32I Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PAFVLAEAMR + H2O
-
 Human immunodeficiency virus 1 PAFVL + AEAMR
-
 ?
PAFVLMEAMR + H2O
-
 Human immunodeficiency virus 1 ?
-
 ?
PARVLAEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + AEAMR
-
 ?
PARVLFEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + FEAMR
-
 ?
PARVLIEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + IEAMR
-
 ?
PARVLLAEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + LAEAMR
-
 ?
PARVLLEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + LEAMR
-
 ?
PARVLMEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + MEAMR
-
 ?
PARVLVEAMR + H2O
-
 Human immunodeficiency virus 1 PARVL + VEAMR
-
 ?
PPAVSLAMTMRR + H2O
-
 Human immunodeficiency virus 1 PPAVS + LAMTMRR
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0667
-
 PARVLAEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.0833
-
 PAFVLAEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.133
-
 PARVLAEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.167
-
 PAFVLAEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.183
-
 PAFVLAEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.2
-
 PARVLAEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.25
-
 PAFVLAEAMR wild-type enzyme Human immunodeficiency virus 1
0.267
-
 PARVLAEAMR wild-type enzyme Human immunodeficiency virus 1
0.317
-
 PAFVLAEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.333
-
 PARVLAEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
0.367
-
 PARVLIEAMR wild-type enzyme Human immunodeficiency virus 1
0.467
-
 PARVLFEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.467
-
 PARVLLEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.5
-
 PARVLVEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.533
-
 PARVLAEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.583
-
 PARVLLEAMR wild-type enzyme Human immunodeficiency virus 1
0.583
-
 PAFVLAEAMR mutant enzyme V82T Human immunodeficiency virus 1
0.6
-
 PARVLIEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.6
-
 PARVLMEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.617
-
 PARVLLEAMR mutant enzyme R8K Human immunodeficiency virus 1
0.633
-
 PARVLLEAMR mutant enzyme V32I Human immunodeficiency virus 1
0.717
-
 PARVLVEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
0.75
-
 PARVLAEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.833
-
 PARVLVEAMR wild-type enzyme Human immunodeficiency virus 1
0.9
-
 PARVLAEAMR mutant enzyme I84V Human immunodeficiency virus 1
0.933
-
 PAFVLMEAMR wild-type enzyme Human immunodeficiency virus 1
1.02
-
 PARVLMEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
1.03
-
 PARVLFEAMR wild-type enzyme Human immunodeficiency virus 1
1.03
-
 PARVLIEAMR mutant enzyme V82T Human immunodeficiency virus 1
1.07
-
 PARVLIEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
1.07
-
 PARVLMEAMR mutant enzyme R8K Human immunodeficiency virus 1
1.1
-
 PARVLFEAMR mutant enzyme R8K Human immunodeficiency virus 1
1.15
-
 PARVLFEAMR mutant enzyme G48V/L90M Human immunodeficiency virus 1
1.17
-
 PARVLIEAMR mutant enzyme R8K Human immunodeficiency virus 1
1.22
-
 PARVLLEAMR mutant enzyme V82T Human immunodeficiency virus 1
1.23
-
 PARVLVEAMR mutant enzyme R8K Human immunodeficiency virus 1
1.28
-
 PARVLMEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
1.3
-
 PARVLMEAMR mutant enzyme I84V Human immunodeficiency virus 1
1.32
-
 PARVLVEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
1.57
-
 PARVLFEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
1.6
-
 PARVLIEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
1.6
-
 PARVLLEAMR mutant enzyme V82T/I84V Human immunodeficiency virus 1
1.73
-
 PARVLFEAMR mutant enzyme V82T Human immunodeficiency virus 1
1.82
-
 PARVLIEAMR mutant enzyme I84V Human immunodeficiency virus 1
1.87
-
 PARVLMEAMR mutant enzyme V82T Human immunodeficiency virus 1
2
-
 PARVLVEAMR mutant enzyme V82T Human immunodeficiency virus 1
2.02
-
 PARVLVEAMR mutant enzyme I84V Human immunodeficiency virus 1
2.22
-
 PARVLLEAMR mutant enzyme I84V Human immunodeficiency virus 1
2.55
-
 PARVLFEAMR mutant enzyme I84V Human immunodeficiency virus 1