Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.12 extracted from

  • Buch, F.; Kaman, W.E.; Bikker, F.J.; Yilamujiang, A.; Mithoefer, A.
    Nepenthesin protease activity indicates digestive fluid dynamics in carnivorous nepenthes plants (2015), PLoS ONE, 10, e0118853 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda Sf9 cells Nepenthes alata
expressed in Spodoptera frugiperda Sf9 cells Nepenthes mirabilis

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride about 70% residual activity at 0.1 mM Nepenthes mirabilis
DMSO about 65% residual activity at 0.1 mM Nepenthes mirabilis
E-64 about 97% residual activity at 0.02 mM Nepenthes mirabilis
pepstatin A almost complete inhibition at 0.1 mM Nepenthes alata
pepstatin A almost complete inhibition at 0.1 mM Nepenthes mirabilis
Phenanthroline about 65% residual activity at 0.1 mM Nepenthes mirabilis

Organism

Organism UniProt Comment Textmining
Nepenthes alata
-
-
-
Nepenthes mirabilis K4ML82
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pitcher fluid
-
 Nepenthes alata
-
pitcher fluid
-
 Nepenthes mirabilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PFU-093 + H2O FITC(Ahx)-L-Val-L-Val-L-Lys-Dbc Nepenthes alata FITC(Ahx)-L-Val + L-Val + L-Lys-Dbc
-
 ?
PFU-093 + H2O FITC(Ahx)-L-Val-L-Val-L-Lys-Dbc Nepenthes mirabilis FITC(Ahx)-L-Val + L-Val + L-Lys-Dbc
-
 ?

Synonyms

Synonyms Comment Organism
Nep II
-
 Nepenthes alata
Nep II
-
 Nepenthes mirabilis
nepenthesin II
-
 Nepenthes alata
nepenthesin II
-
 Nepenthes mirabilis

Expression

Organism Comment Expression
Nepenthes alata proteolytic activity in pitcher fluid is induced by the phytohormone jasmonic acid but not by salicylic acid up
Nepenthes mirabilis proteolytic activity in pitcher fluid is induced by the phytohormone jasmonic acid but not by salicylic acid up