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Literature summary for 3.4.23.1 extracted from

  • Luo, Q.; Chen, D.; Boom, R.M.; Janssen, A.E.M.
    Revisiting the enzymatic kinetics of pepsin using isothermal titration calorimetry (2018), Food Chem., 268, 94-100 .
    View publication on PubMed
Search for more literature for 3.4.23.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
0.2
-
 bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
0.2
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
0.26
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
0.33
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
0.37
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
0.4
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
0.4
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
0.7
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
0.8
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
1.1
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gastric mucosa
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Gly-Phe + Phe 3-(4-pyridyl)propyl ester
-
 ?
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Phe + Phe 3-(4-pyridyl)propyl ester
-
 ?
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Phe + Phe 3-(4-pyridyl)propyl ester
-
 ?
benzyloxycarbonyl-Phe-Phe-OEt + H2O
-
 Sus scrofa benzyloxycarbonyl-Phe + Phe-OEt
-
 ?
Bovine serum albumin + H2O
-
 Sus scrofa ?
-
 ?
Z-GGFF-OP4P + H2O
-
 Sus scrofa benzyloxycarbonyl-GGF + Phe 3-(4-pyridyl)propyl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 34600, calculated from amino acid sequence Sus scrofa

Synonyms

Synonyms Comment Organism
pepsin
-
 Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
0.1
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
0.11
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
0.13
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
0.49
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
0.74
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
2.2
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.1
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
4.44
-
 bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
56.5
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
71.8
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.31
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
0.35
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
0.39
-
 benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
0.7
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
2
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
2.65
-
 benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
3.7
-
 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
7.8
-
 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
22.1
-
 bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
70.6
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
180
-
 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa