Application | Comment | Organism |
---|---|---|
medicine | the nsp2pro N-terminal domain is a novel cysteine protease fold. the C-terminal domain displays structural similarity to S-adenosyl-L-methionine-dependent RNA methyltransferases. This structure will significantly aid drug discovery and development efforts to combat Venezuelan equine encephalitis alphavirus and related viruses | Venezuelan equine encephalitis virus |
Cloned (Comment) | Organism |
---|---|
DNA coding for nsP2pro (residues Met457-Cys794) amplified and cloned into vector pETBlue1 T7. Expression in Tuner DE3 (pLacI) Escherichia coli cells | Venezuelan equine encephalitis virus |
Crystallization (Comment) | Organism |
---|---|
at 2.45 A resolution. Belongs to space group P212121. N-terminal domain (Asp468 to Asn603) contains the catalytic dyad formed by Cys477 and His546, organized in a protein fold. C-terminal domain (Arg604 to Ser793) contributes to substrate recognition and regulates the structure of the substrate binding cleft | Venezuelan equine encephalitis virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Venezuelan equine encephalitis virus | P27282 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, more than 99% pure | Venezuelan equine encephalitis virus |
Synonyms | Comment | Organism |
---|---|---|
nsP2 protease | - |
Venezuelan equine encephalitis virus |