Cloned (Comment) | Organism |
---|---|
expression as a GST-Ulp2 fusion protein by Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by phenylmethylsulfonyl fluoride or the Dub-specific inhibitor ubiquitin aldehyde | Saccharomyces cerevisiae | |
N-ethylmaleimide | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Smt3-modified protein conjugate + H2O | Saccharomyces cerevisiae | Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development | Smt3 + protein | - |
? | |
Smt3-precursor + H2O | Saccharomyces cerevisiae | Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1 | SMT3 + peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40537 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Smt3-modified protein conjugate + H2O | Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development | Saccharomyces cerevisiae | Smt3 + protein | - |
? | |
Smt3-modified protein conjugate + H2O | Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates | Saccharomyces cerevisiae | Smt3 + protein | - |
? | |
Smt3-precursor + H2O | Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1 | Saccharomyces cerevisiae | SMT3 + peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMT4 | - |
Saccharomyces cerevisiae |
Ulp2 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of ULP2 results in a diverse set of aberrations, but the gene is not essential. In cells lacking Ulp2, specific Smt3-protein conjugates accumulate, and the conjugate pattern is distinct from that observed in a ulp1ts strain, which is defective for a distantly related Smt3-specific protease, Ulp1. The ulp2D mutant exhibits a pleiotropic phenotype that includes temperature-sensitive growth, abnormal cell morphology, decreased plasmid and chromosome stability, and a severe sporulation defect. The mutant is also hypersensitive to DNA damaging agents, hydroxyurea, and benomyl | Saccharomyces cerevisiae |